Browsing Ciències Bàsiques by Title
Now showing items 23-42 of 65
-
Engineering a genome-reduced bacterium to eliminate Staphylococcus aureus biofilms in vivo
(Molecular Systems Biology, 2021)Bacteria present a promising delivery system for treating human diseases. Here, we engineered the genome-reduced human lung pathogen Mycoplasma pneumoniae as a live biotherapeutic to treat biofilm-associated ... -
Evolutionary footprints reveal insights into plant MicroRNA biogenesis
(The Plant Cell, 2017)MicroRNAs (miRNAs) are endogenous small RNAs that recognize target sequences by base complementarity and play a role in the regulation of target gene expression. They are processed from longer precursor ... -
Functional characterization of a novel non-coding mutation “Ghent +49A > G” in the iron-responsive element of L-ferritin causing hereditary hyperferritinaemia-cataract syndrome
(Scientific Reports, 2017)Hereditary hyperferritinaemia-cataract syndrome (HHCS) is a rare disorder usually caused by heterozygous mutations in the iron-responsive element (IRE) in the 5′ untranslated region (5′UTR) of the ... -
Global signalling network analysis of luminal T47D breast cancer cells in response to progesterone
(Frontiers in Endocrinology, 2022)Background: Breast cancer cells enter into the cell cycle following progestin exposure by the activation of signalling cascades involving a plethora of enzymes, transcription factors and co-factors that ... -
Hereditary hyperferritinemia cataract syndrome: ferritin L gene and physiopathology behind the disease - report of new cases
(International Journal of Molecular Sciences, 2021)Hereditary hyperferritinemia-cataract syndrome (HHCS) is a rare disease characterized by high serum ferritin levels, congenital bilateral cataracts, and the absence of tissue iron overload. This disorder ... -
Hormone-induced repression of genes requires BRG1-mediated H1.2 deposition at target promoters
(The EMBO Journal, 2016)Eukaryotic gene regulation is associated with changes in chromatin compaction that modulate access to DNA regulatory sequences relevant for transcriptional activation or repression. Although much is ... -
Identification of key sequence features required for microRNA biogenesis in plants
(Nature Communications, 2020)MicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop ... -
Identification of new microRNA-regulated genes by conserved targeting in plant species
(Nucleic Acids Research, 2012)MicroRNAs (miRNAs) are major regulators of gene expression in multicellular organisms. They recognize their targets by sequence complementarity and guide them to cleavage or translational arrest. It is ... -
Improved dynamical particle swarm optimization method for structural dynamics
(Mathematical Problems in Engineering, 2019)A methodology to the multiobjective structural design of buildings based on an improved particle swarm optimization algorithm is presented, which has proved to be very efficient and robust in nonlinear ... -
Improving signal/noise resolution in single-molecule experiments using molecular constructs with short handles
(Biophysical Journal, 2011)We investigate unfolding/folding force kinetics in DNA hairpins exhibiting two and three states with newly designed short dsDNA handles (29 bp) using optical tweezers. We show how the higher stiffness ... -
Influence of bone-level dental implants placement and of cortical thickness on osseointegration: in silico and in vivo analyses
(Journal of Clinical Medicine, 2022)The purpose of this research is to study the biomechanical response of dental implants in bone-level type locations, 0.5 mm above and below the bone level. In addition, the influence of the thickness ... -
Interexaminer reliability and validity of quantity of cervical mobility during online dynamic inspection
(Diagnostics, 2022)Background: Physical therapists routinely measure range of motion (ROM) of cervical spine. The reliability of the cervical range of motion (CROM) device has been demonstrated in several studies, but ... -
Investigating the thermodynamics of small biosystems with optical tweezers
(Physica E: Low-dimensional Systems and Nanostructures, 2010)We present two examples of how single-molecule experimental techniques applied to biological systems can give insight into problems within the scope of equilibrium and nonequilibrium mesoscopic ... -
Iron Regulation and the Cell Cycle: identification of an iron-responsive element in the 3′-untranslated region of human cell division cycle 14a mrna by a refined microarray-based screening strategy
(The Journal of Biological Chemistry, 2006)Iron regulatory proteins (IRPs) 1 and 2 post-transcriptionally control mammalian iron homeostasis by binding to iron-responsive elements (IREs), conserved RNA stem-loop structures located in the 5′- or ... -
LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions
(Microbial Biotechnology, 2021)The removal of unwanted genetic material is a key aspect in many synthetic biology efforts and often requires preliminary knowledge of which genomic regions are dispensable. Typically, these efforts are ... -
Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
(Materials Today Bio, 2021-09)Biomimetic calcium-deficient hydroxyapatite (CDHA) as a bioactive material exhibits exceptional intrinsic osteoinductive and osteogenic properties because of its nanostructure and composition, which ... -
Measurement of work in single-molecule pulling experiments
(The Journal of Chemical Physics, 2009)A main goal of single-molecule experiments is to evaluate equilibrium free energy differences by applying fluctuation relations to repeated work measurements along irreversible processes. We quantify ... -
MetaPhOrs 2.0: integrative, phylogeny-based inference of orthology and paralogy across the tree of life
(Nucleic Acids Research, 2020)Inferring homology relationships across genes in different species is a central task in comparative genomics. Therefore, a large number of resources and methods have been developed over the years. Some ... -
Modeling cell adhesion and proliferation: a cellular-automata based approach
(Advanced Modeling and Simulation in Engineering Sciences, 2015)Background: Cell adhesion is a process that involves the interaction between the cell membrane and another surface, either a cell or a substrate. Unlike experimental tests, computer models can simulate ... -
Multiple ion scaffold-based delivery platform for potential application in early stages of bone regeneration
(Materials, 2021)Bone has the intrinsic capacity to regenerate itself, as long as the damage is small, through the sequential stimulation of specific phases, such as angiogenesis followed by osteogenesis. However, when ...