Identification of new microRNA-regulated genes by conserved targeting in plant species
Author
Chorostecki, Uciel
Crosa, Valeria A.
Lodeyro, Anabella F.
Bologna, Nicolás G.
Martin, Ana P.
Carrillo, Néstor
Schommer, Carla
Palatnik, Javier F.
Publication date
2012ISSN
0305-1048
Abstract
MicroRNAs (miRNAs) are major regulators of gene expression in multicellular organisms. They recognize their targets by sequence complementarity and guide them to cleavage or translational arrest. It is generally accepted that plant miRNAs have extensive complementarity to their targets and their prediction usually relies on the use of empirical parameters deduced from known miRNA–target interactions. Here, we developed a strategy to identify miRNA targets which is mainly based on the conservation of the potential regulation in different species. We applied the approach to expressed sequence tags datasets from angiosperms. Using this strategy, we predicted many new interactions and experimentally validated previously unknown miRNA targets in Arabidopsis thaliana . Newly identified targets that are broadly conserved include auxin regulators, transcription factors and transporters. Some of them might participate in the same pathways as the targets known before, suggesting that some miRNAs might control different aspects of a biological process. Furthermore, this approach can be used to identify targets present in a specific group of species, and, as a proof of principle, we analyzed Solanaceae -specific targets. The presented strategy can be used alone or in combination with other approaches to find miRNA targets in plants.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
5 - Natural Sciences
Keywords
Genètica
Genética
Genetics
Pages
11
Publisher
Oxford University Press
Collection
40; 18
Is part of
Nucleic Acids Research
Citation
Chorostecki, Uciel; Crosa, Valeria A.; Lodeyro, Anabella F. [et al.]. Identification of new microRNA-regulated genes by conserved targeting in plant species. Nucleic Acids Research, 2012, 40(18), p. 8893-8904. Disponible en: <https://academic.oup.com/nar/article/40/18/8893/2411305?login=true>. Fecha de acceso: 7 feb. 2024. DOI: 10.1093/nar/gks625
This item appears in the following Collection(s)
- Ciències Bàsiques [65]
Rights
© The Author(s) 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.