Listando Ciències Bàsiques por fecha de publicación
Mostrando ítems 1-20 de 94
-
Long-term expression of erythropoietin from myoblasts immobilized in biocompatible and neovascularized microcapsules
(Molecular Therapy, 2005)The present paper investigates the long-term functionality of an ex vivo gene therapy approach based on cell microencapsulation for the continuous delivery of erythropoietin (EPO) without implementation ... -
Iron Regulation and the Cell Cycle: identification of an iron-responsive element in the 3′-untranslated region of human cell division cycle 14a mrna by a refined microarray-based screening strategy
(The Journal of Biological Chemistry, 2006)Iron regulatory proteins (IRPs) 1 and 2 post-transcriptionally control mammalian iron homeostasis by binding to iron-responsive elements (IREs), conserved RNA stem-loop structures located in the 5′- or ... -
Differential effects of muscarinic receptor blockade in prelimbic cortex on acquisition and memory formation of an odor-reward task
(Learning Memory, 2007)The present experiments determined the consequences of blocking muscarinic cholinergic receptors of the prelimbic (PL) cortex in the acquisition and retention of an odor-reward associative task. Rats ... -
Muscarinic receptor blockade in ventral hippocampus and prelimbic cortex impairs memory for socially transmitted food preference
(Hippocampus, 2008)Acetylcholine is involved in learning and memory and, particularly, in olfactory tasks, but reports on its specific role in consolidation processes are somewhat controversial. The present experiment ... -
2P-111 Stochastic resonance in DNA hairpins(The 46th Annual Meeting of the Biophysical Society of Japan)
(Seibutsu Butsuri, 2008) -
Work distributions in the T = 0 random field Ising model
(Physical Review E, 2008)We perform a numerical study of the three-dimensional random-field Ising model at T= 0. We compare work distributions along metastable trajectories obtained with the single-spin flip dynamics with the ... -
Statistical properties of metastable intermediates in DNA unzipping
(Physical Review Letters, 2009)We unzip DNA molecules using optical tweezers and determine the sizes of the cooperatively unzipping and zipping regions separating consecutive metastable intermediates along the unzipping pathway. Sizes ... -
Muscarinic transmission in the basolateral amygdala is necessary for the acquisition of socially transmitted food preferences in rats
(Neurobiology of Learning and Memory, 2009)We examined the involvement of muscarinic receptors in the basolateral amygdala (BLA) in the social transmission of food preference (STFP) learning by assessing the effects of scopolamine (20 μg/side), ... -
Dynamic force spectroscopy of DNA hairpins: II. Irreversibility and dissipation
(Journal of Statistical Mechanics: Theory and Experiment, 2009)We investigate irreversibility and dissipation in single molecules that cooperatively fold/unfold in a two-state manner under the action of mechanical force. We apply path thermodynamics to derive ... -
Measurement of work in single-molecule pulling experiments
(The Journal of Chemical Physics, 2009)A main goal of single-molecule experiments is to evaluate equilibrium free energy differences by applying fluctuation relations to repeated work measurements along irreversible processes. We quantify ... -
Dynamic force spectroscopy of DNA hairpins: I. Force kinetics and free energy landscapes
(Journal of Statistical Mechanics: Theory and Experiment, 2009)We investigate the thermodynamics and kinetics of DNA hairpins that fold/unfold under the action of applied mechanical force. We introduce the concept of the molecular free energy landscape and derive ... -
Single-molecule derivation of salt dependent base-pair free energies in DNA
(Proceedings of the National Academy of Sciences., 2010)Accurate knowledge of the thermodynamic properties of nucleic acids is crucial to predicting their structure and stability. To date most measurements of base-pair free energies in DNA are obtained in ... -
Investigating the thermodynamics of small biosystems with optical tweezers
(Physica E: Low-dimensional Systems and Nanostructures, 2010)We present two examples of how single-molecule experimental techniques applied to biological systems can give insight into problems within the scope of equilibrium and nonequilibrium mesoscopic ... -
Effects of muscarinic receptor antagonism in the basolateral amygdala on two-way active avoidance
(Experimental Brain Research, 2011)The aim of the present study was to investigate whether the blockade of muscarinic receptors (mRs) in the basolateral amygdala (BLA), which receives important cholinergic inputs related to avoidance ... -
Multisensory information facilitates reaction speed by enlarging activity difference between superior colliculus hemispheres in rats
(PLoS ONE, 2011)Animals can make faster behavioral responses to multisensory stimuli than to unisensory stimuli. The superior colliculus (SC), which receives multiple inputs from different sensory modalities, is ... -
Improving signal/noise resolution in single-molecule experiments using molecular constructs with short handles
(Biophysical Journal, 2011)We investigate unfolding/folding force kinetics in DNA hairpins exhibiting two and three states with newly designed short dsDNA handles (29 bp) using optical tweezers. We show how the higher stiffness ... -
Identification of new microRNA-regulated genes by conserved targeting in plant species
(Nucleic Acids Research, 2012)MicroRNAs (miRNAs) are major regulators of gene expression in multicellular organisms. They recognize their targets by sequence complementarity and guide them to cleavage or translational arrest. It is ... -
Single-molecule stochastic resonance
(Physical Review X, 2012)Stochastic resonance (SR) is a well-known phenomenon in dynamical systems. It consists of the amplification and optimization of the response of a system assisted by stochastic (random or probabilistic) ... -
Multiple RNA recognition patterns during microRNA biogenesis in plants
(Genome Research, 2013)MicroRNAs (miRNAs) derive from longer precursors with fold-back structures. While animal miRNA precursors have homogenous structures, plant precursors comprise a collection of fold-backs with variable ... -
Counter-propagating dual-trap optical tweezers based on linear momentum conservation
(Review of Scientific Instruments, 2013)We present a dual-trap optical tweezers setup which directly measures forces using linear momentum conservation. The setup uses a counter-propagating geometry, which allows momentum measurement on each ...