Immobilization of BMP-2 and VEGF within multilayered polydopamine-coated scaffolds and the resulting osteogenic and angiogenic synergy of co-cultured human mesenchymal stem cells and human endothelial progenitor cells
Autor/a
Godoy-Gallardo, Maria
Portolés-Gil, Núria
López-Periago, Ana M.
Domingo, Concepción
Hosta-Rigau, Leticia
Fecha de publicación
2020ISSN
1422-0067
Resumen
We have previously reported the fabrication of a polycaprolactone and hydroxyapatite composite scaffold incorporating growth factors to be used for bone regeneration. Two growth factors were incorporated employing a multilayered coating based on polydopamine (PDA). In particular, Bone morphogenetic protein-2 (BMP-2) was bound onto the inner PDA layer while vascular endothelial growth factor (VEGF) was immobilized onto the outer one. Herein, the in vitro release of both growth factors is evaluated. A fastest VEGF delivery followed by a slow and more sustained release of BMP-2 was demonstrated, thus fitting the needs for bone tissue engineering applications. Due to the relevance of the crosstalk between bone-promoting and vessel-forming cells during bone healing, the functionalized scaffolds are further assessed on a co-culture setup of human mesenchymal stem cells and human endothelial progenitor cells. Osteogenic and angiogenic gene expression analysis indicates a synergistic effect between the growth factor-loaded scaffolds and the co-culture conditions. Taken together, these results indicate that the developed scaffolds hold great potential as an efficient platform for bone-tissue applications.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
61 - Medicina
616.7 - Patología de los órganos de la locomoción. Sistema locomotor y esquelético
Palabras clave
Angiogènesi
Enginyeria del teixit ossi
Coentrega
Factors de creixement
Multicapa
Osteogènesi
Polidopamina
Bastides
Angiogénesis
Ingeniería de tejidos óseos
Co-entrega
Factores de crecimiento
Multicapas
Osteogénesis
Polidopamina
Andamios
Angiogenesis
Bone tissue engineering
Co-delivery
Growth factors
Multilayers
Osteogenesis
Polydopamine
Scaffolds
Páginas
25
Publicado por
MDPI
Colección
21;17
Publicado en
International Journal of Molecular Sciences
Citación
Godoy-Gallardo, Maria; Portolés-Gil, Núria; López-Periago, Ana M. [et al.]. Immobilization of BMP-2 and VEGF within multilayered polydopamine-coated scaffolds and the resulting osteogenic and angiogenic synergy of co-cultured human mesenchymal stem cells and human endothelial progenitor cells. International Journal of Molecular Sciences, 2020, 21(17), 6418. Disponible en: <https://www.mdpi.com/1422-0067/21/17/6418>. Fecha de acceso: 17 ene. 2022. DOI: 10.3390/ijms21176418
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