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dc.contributor.authorGodoy-Gallardo, Maria
dc.contributor.authorPortolés-Gil, Núria
dc.contributor.authorLópez-Periago, Ana M.
dc.contributor.authorDomingo, Concepción
dc.contributor.authorHosta-Rigau, Leticia
dc.date.accessioned2022-01-17T13:23:16Z
dc.date.available2022-01-17T13:23:16Z
dc.date.issued2020
dc.identifier.citationGodoy-Gallardo, Maria; Portolés-Gil, Núria; López-Periago, Ana M. [et al.]. Immobilization of BMP-2 and VEGF within multilayered polydopamine-coated scaffolds and the resulting osteogenic and angiogenic synergy of co-cultured human mesenchymal stem cells and human endothelial progenitor cells. International Journal of Molecular Sciences, 2020, 21(17), 6418. Disponible en: <https://www.mdpi.com/1422-0067/21/17/6418>. Fecha de acceso: 17 ene. 2022. DOI: 10.3390/ijms21176418ca
dc.identifier.issn1422-0067ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/3067
dc.description.abstractWe have previously reported the fabrication of a polycaprolactone and hydroxyapatite composite scaffold incorporating growth factors to be used for bone regeneration. Two growth factors were incorporated employing a multilayered coating based on polydopamine (PDA). In particular, Bone morphogenetic protein-2 (BMP-2) was bound onto the inner PDA layer while vascular endothelial growth factor (VEGF) was immobilized onto the outer one. Herein, the in vitro release of both growth factors is evaluated. A fastest VEGF delivery followed by a slow and more sustained release of BMP-2 was demonstrated, thus fitting the needs for bone tissue engineering applications. Due to the relevance of the crosstalk between bone-promoting and vessel-forming cells during bone healing, the functionalized scaffolds are further assessed on a co-culture setup of human mesenchymal stem cells and human endothelial progenitor cells. Osteogenic and angiogenic gene expression analysis indicates a synergistic effect between the growth factor-loaded scaffolds and the co-culture conditions. Taken together, these results indicate that the developed scaffolds hold great potential as an efficient platform for bone-tissue applications.en
dc.format.extent25ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofInternational Journal of Molecular Sciencesca
dc.relation.ispartofseries21;17
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.otherAngiogènesica
dc.subject.otherEnginyeria del teixit ossica
dc.subject.otherCoentregaca
dc.subject.otherFactors de creixementca
dc.subject.otherMulticapaca
dc.subject.otherOsteogènesica
dc.subject.otherPolidopaminaca
dc.subject.otherBastidesca
dc.subject.otherAngiogénesises
dc.subject.otherIngeniería de tejidos óseoses
dc.subject.otherCo-entregaes
dc.subject.otherFactores de crecimientoes
dc.subject.otherMulticapases
dc.subject.otherOsteogénesises
dc.subject.otherPolidopaminaes
dc.subject.otherAndamioses
dc.subject.otherAngiogenesisen
dc.subject.otherBone tissue engineeringen
dc.subject.otherCo-deliveryen
dc.subject.otherGrowth factorsen
dc.subject.otherMultilayersen
dc.subject.otherOsteogenesisen
dc.subject.otherPolydopamineen
dc.subject.otherScaffoldsen
dc.titleImmobilization of BMP-2 and VEGF within multilayered polydopamine-coated scaffolds and the resulting osteogenic and angiogenic synergy of co-cultured human mesenchymal stem cells and human endothelial progenitor cellsen
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc61ca
dc.subject.udc616.7ca
dc.identifier.doihttps://dx.doi.org/10.3390/ijms21176418ca


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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/