Mimicking bone extracellular matrix: integrin-binding peptidomimetics enhance osteoblast-like cells adhesion, proliferation, and differentiation on titanium
Author
Fraioli, Roberta
Rechenmacher, Florian
Neubauer, Stefanie
Manero Planella, José María
Gil Mur, Francisco Javier
Kessler, Horst
Mas Moruno, Carlos
Publication date
2015-04-01ISSN
0927-7765
Abstract
Interaction between the surface of implants and biological tissues is a key aspect of biomaterials research. Apart from fulfilling the non-toxicity and structural requirements, synthetic materials are asked to direct cell response, offering engineered cues that provide specific instructions to cells. This work explores the functionalization of titanium with integrin-binding peptidomimetics as a novel and powerful strategy to improve the adhesion, proliferation and differentiation of osteoblast-like cells to implant materials. Such biomimetic strategy aims at targeting integrins αvβ3 and α5β1, which are highly expressed on osteoblasts and are essential for many fundamental functions in bone tissue development. The successful grafting of the bioactive molecules on titanium is proven by contact angle measurements, X-ray photoelectron spectroscopy and fluorescent labeling. Early attachment and spreading of cells are statistically enhanced by both peptidomimetics compared to unmodified titanium, reaching values of cell adhesion comparable to those obtained with full-length extracellular matrix proteins. Moreover, an increase in alkaline phosphatase activity, and statistically higher cell proliferation and mineralization are observed on surfaces coated with the peptidomimetics. This study shows an unprecedented biological activity for low-molecular-weight ligands on titanium, and gives striking evidence of the potential of these molecules to foster bone regeneration on implant materials.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
57 - Biological sciences in general
61 - Medical sciences
Keywords
Osteointegració
Materials biomèdics
Ossos -- Regeneració
Osteointegración
Materiales biomédicos
Huesos -- Regeneración
Osteointegration
Biomedical materials
Bones -- Regeneration
Pages
10
Publisher
Elsevier
Collection
128;
Is part of
Colloids and Surfaces B: Biointerfaces
Citation
Fraioli, Roberta; Rechenmacher, Florian; Neubauer, Stefanie [et al.]. Mimicking bone extracellular matrix: integrin-binding peptidomimetics enhance osteoblast-like cells adhesion, proliferation, and differentiation on titanium. Colloids and Surfaces B: Biointerfaces, 2015, 128, p. 191-200. Disponible en: <https://www.sciencedirect.com/science/article/pii/S0927776515000168?via%3Dihub>. Fecha de acceso: 10 may. 2021. DOI: 10.1016/j.colsurfb.2014.12.057
Grant agreement number
info:eu-repo/grantAgreement/EC/FP7/321985
info:eu-repo/grantAgreement/MINECO/6PN/MAT2012-30706
This item appears in the following Collection(s)
- Ciències de la Salut [740]
Rights
Under a Creative Commons license
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/