Dynamic force spectroscopy of DNA hairpins: II. Irreversibility and dissipation
Author
Manosas, M.
Mossa, A.
Forns, N.
Huguet Casades, Josep Maria
Ritort, F.
Publication date
2009ISSN
1742-5468
Abstract
We investigate irreversibility and dissipation in single molecules that cooperatively fold/unfold in a two-state manner under the action of mechanical force. We apply path thermodynamics to derive analytical expressions for the average dissipated work and the average hopping number in two-state systems. It is shown how these quantities only depend on two parameters that characterize the folding/unfolding kinetics of the molecule: the fragility and the coexistence hopping rate. The latter has to be rescaled to take into account the appropriate experimental set-up. Finally we carry out pulling experiments with optical tweezers in a specifically designed DNA hairpin that shows two-state cooperative folding. We then use these experimental results to validate our theoretical predictions.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
57 - Biological sciences in general
Keywords
ADN
DNA
Pages
Desconocido
Publisher
IOP Publishing
Collection
2009
Is part of
Journal of Statistical Mechanics: Theory and Experiment
Citation
Manosas, M.; Mossa, A.; Forns, N. [et al.]. Dynamic force spectroscopy of DNA hairpins: II. Irreversibility and dissipation. Journal of Statistical Mechanics: Theory and Experiment, 2009, 2009, P02061. Disponible en: <https://iopscience.iop.org/article/10.1088/1742-5468/2009/02/P02061/pdf>. Fecha de acceso: 18 mar. 2024. DOI: 10.1088/1742-5468/2009/02/P02061
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- Ciències Bàsiques [65]
Rights
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