Browsing by Subject "ADN"
Now showing items 1-13 of 13
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Aberrantly spliced mRNAs of the 3-hydroxy-3-methylglutaryl coenzyme A lyase (HL) gene with a donor splice-site point mutation produce hereditary HL deficiency
(Journal of Lipid Research, 1996-11)A novel point mutation in the 3-hydroxy-3methyl-glutaryl coenzyme A lyase gene was found in a Turkish patient with homozygous 3-hydroxy-3-methylglutaric acidemia. Amplification by RT-PCR of the mRNA ... -
ADP-ribose–derived nuclear ATP synthesis by NUDIX5 is required for chromatin remodeling
(Science, 2016)Key nuclear processes in eukaryotes, including DNA replication, repair, and gene regulation, require extensive chromatin remodeling catalyzed by energy-consuming enzymes. It remains unclear how the ATP ... -
CDK-mediated Yku80 phosphorylation regulates the balance between non-homologous end joining (NHEJ) and homologous directed recombination (HDR)
(Journal of Molecular Biology, 2020)There are two major pathways for repairing DNA double-strand breaks (DSBs): homologous directed recombination (HDR) and non-homologous end-joining (NHEJ). While NHEJ functions throughout the cell cycle, ... -
Dynamic force spectroscopy of DNA hairpins: I. Force kinetics and free energy landscapes
(Journal of Statistical Mechanics: Theory and Experiment, 2009)We investigate the thermodynamics and kinetics of DNA hairpins that fold/unfold under the action of applied mechanical force. We introduce the concept of the molecular free energy landscape and derive ... -
Dynamic force spectroscopy of DNA hairpins: II. Irreversibility and dissipation
(Journal of Statistical Mechanics: Theory and Experiment, 2009)We investigate irreversibility and dissipation in single molecules that cooperatively fold/unfold in a two-state manner under the action of mechanical force. We apply path thermodynamics to derive ... -
Hormone-induced repression of genes requires BRG1-mediated H1.2 deposition at target promoters
(The EMBO Journal, 2016)Eukaryotic gene regulation is associated with changes in chromatin compaction that modulate access to DNA regulatory sequences relevant for transcriptional activation or repression. Although much is ... -
Improving signal/noise resolution in single-molecule experiments using molecular constructs with short handles
(Biophysical Journal, 2011)We investigate unfolding/folding force kinetics in DNA hairpins exhibiting two and three states with newly designed short dsDNA handles (29 bp) using optical tweezers. We show how the higher stiffness ... -
The IronChip evaluation package: a package of perl modules for robust analysis of custom microarrays
(BMC Bioinformatics, 2010)Background: Gene expression studies greatly contribute to our understanding of complex relationships in gene regulatory networks. However, the complexity of array design, production and manipulations ... -
Una lectura del descubrimiento del ADN a través de la teoría paradigmática de Thomas Kuhn
(2022)El propósito del presente trabajo es hacer una lectura de la teoría descrita por Thomas Kuhn, en su libro titulado La Estructura de las revoluciones científicas, aplicada a la historia del descubrimiento ... -
Nucleosome architecture throughout the cell cycle
(Scientific Reports, 2016-01-28)Nucleosomes provide additional regulatory mechanisms to transcription and DNA replication by mediating the access of proteins to DNA. During the cell cycle chromatin undergoes several conformational ... -
The transcriptional network activated by Cln3 cyclin at the G1-to-S transition of the yeast cell cycle
(Genome Biology, 2010-06-23)Background: The G1-to-S transition of the cell cycle in the yeast Saccharomyces cerevisiae involves an extensive transcriptional program driven by transcription factors SBF (Swi4-Swi6) and MBF (Mbp1-Swi6). ... -
Unliganded progesterone receptor governs estrogen receptor gene expression by regulating DNA methylation in breast cancer cells
(Cancers, 2018)Breast cancer prognosis and response to endocrine therapy strongly depends on the expression of the estrogen and progesterone receptors (ER and PR, respectively). Although much is known about ERα gene ... -
Unravelling the encapsulation of DNA and other biomolecules in HAp microcalcifications of human breast cancer tissues by raman imaging
(Cancers, 2021)Microcalcifications are detected through mammography screening and, depending on their morphology and distribution (BI-RADS classification), they can be considered one of the first indicators of suspicious ...