Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays

Sanchez, Mayka; Galy, Bruno; Hentze, Matthias W.; Muckenthaler, Martina U.

dc.contributor.author	Sanchez, Mayka
dc.contributor.author	Galy, Bruno
dc.contributor.author	Hentze, Matthias W.
dc.contributor.author	Muckenthaler, Martina U.
dc.date.accessioned	2024-01-31T14:15:13Z
dc.date.available	2024-01-31T14:15:13Z
dc.date.issued	2007
dc.identifier.citation	Sanchez, Mayka; Galy, Bruno; Hentze, Matthias W. [et al.]. Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays. Nature Protocols, 2007, 2, p. 2033–2042. Disponible en: <https://www.nature.com/articles/nprot.2007.293>. Fecha de acceso: 31 ene. 2024. DOI: 10.1038/nprot.2007.293	ca
dc.identifier.uri	http://hdl.handle.net/20.500.12328/3997
dc.description.abstract	RNA-binding proteins (RBPs) frequently regulate the post-transcriptional fate of target mRNAs. To identify novel target mRNAs of RBPs, we incubate total RNA with recombinant RBP and immunoselect the messenger-ribonucleoproteins using a specific anti-RBP antibody. The mRNA composition of the supernatant and/or immunoprecipitated fraction is analyzed using dual-color microarrays in comparison with control reaction. From start to finish, the protocol takes approximately 6 d.	ca
dc.format.extent	9	ca
dc.language.iso	eng	ca
dc.publisher	Springer Nature	ca
dc.relation.ispartof	Nature Protocols	ca
dc.relation.ispartofseries	2
dc.subject.other	Medicina	ca
dc.subject.other	Medicine	ca
dc.title	Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays	ca
dc.type	info:eu-repo/semantics/article	ca
dc.description.version	info:eu-repo/semantics/publishedVersion	ca
dc.rights.accessLevel	info:eu-repo/semantics/openAccess
dc.embargo.terms	cap	ca
dc.subject.udc	61	ca
dc.identifier.doi	https://dx.doi.org/10.1038/nprot.2007.293	ca

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