Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays
Author
Sanchez, Mayka
Galy, Bruno
Hentze, Matthias W.
Muckenthaler, Martina U.
Publication date
2007Abstract
RNA-binding proteins (RBPs) frequently regulate the post-transcriptional fate of target mRNAs. To identify novel target mRNAs of RBPs, we incubate total RNA with recombinant RBP and immunoselect the messenger-ribonucleoproteins using a specific anti-RBP antibody. The mRNA composition of the supernatant and/or immunoprecipitated fraction is analyzed using dual-color microarrays in comparison with control reaction. From start to finish, the protocol takes approximately 6 d.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Medicina
Medicine
Pages
9
Publisher
Springer Nature
Collection
2
Is part of
Nature Protocols
Citation
Sanchez, Mayka; Galy, Bruno; Hentze, Matthias W. [et al.]. Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays. Nature Protocols, 2007, 2, p. 2033–2042. Disponible en: <https://www.nature.com/articles/nprot.2007.293>. Fecha de acceso: 31 ene. 2024. DOI: 10.1038/nprot.2007.293
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- Ciències de la Salut [738]