ADP-ribose–derived nuclear ATP synthesis by NUDIX5 is required for chromatin remodeling
Author
Publication date
2016ISBN
0036-8075
ISSN
Beato
Abstract
Key nuclear processes in eukaryotes, including DNA replication, repair, and gene regulation, require extensive chromatin remodeling catalyzed by energy-consuming enzymes. It remains unclear how the ATP demands of such processes are met in response to rapid stimuli. We analyzed this question in the context of the massive gene regulation changes induced by progestins in breast cancer cells and found that ATP is generated in the cell nucleus via the hydrolysis of poly(ADP-ribose) to ADP-ribose. In the presence of pyrophosphate, ADP-ribose is used by the pyrophosphatase NUDIX5 to generate nuclear ATP. The nuclear source of ATP is essential for hormone-induced chromatin remodeling, transcriptional regulation, and cell proliferation.
Document Type
Article
Document version
Submitted version
Language
English
Subject (CDU)
57 - Biological sciences in general
Keywords
Pages
36
Publisher
American Association for the Advancement of Science
Collection
352; 6290
Is part of
Science
Citation
Wright, Roni H. G.; LeDily, Francois; Soronellas, Daniel [et al.]. ADP-ribose–derived nuclear ATP synthesis by NUDIX5 is required for chromatin remodeling. Science, 2016, 352(6290), p. 1221-1225. Disponible en: <https://www.science.org/doi/10.1126/science.aad9335<. Fecha de acceso: 26 ene. 2022. DOI: 10.1126/science.aad9335
Note
We wish to thank Harm H. Kampinga, Department of Cell Biology, RuG & UMC Groningen, The Netherlands for the nuclear, cytoplasmic and mitochondrial targeted luciferase expressing plasmids, and Hiromi Imamura, The Hakubi Center for Advanced Research & Graduate School of Biostudies, Kyoto University, Japan for the Ateam ATP/ADP expressing plasmids. We also thank Sara Capdevila, and Juan Martin Caballero for their support with IVIS Bioluminescent imaging equipment, the CRG Microarray, Proteomics and Microscopy Facilities for expert help and Juan Valcarcel, CRG, for advice with the manuscript. We acknowledge financial support from the Spanish MEC (CSD2006-00049; BMC 2003-02902 and 2010-15313) and the Catalan Government (AGAUR).
This item appears in the following Collection(s)
- Ciències Bàsiques [94]
Rights
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