MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation
Author
Bisceglie, Lavinia
Hopp, Ann-Katrin
Gunasekera, Kapila
Wright, Roni H.G.
Le Dily, François
Vidal, Enrique
Dall'Agnese, Alessandra
Caputo, Luca
Nicoletti, Chiara
Puri, Pier Lorenzo
Beato, Miguel
Hottiger, Michael O.
Publication date
2021ISSN
2589-0042
Abstract
While protein ADP-ribosylation was reported to regulate differentiation and dedifferentiation, it has so far not been studied during transdifferentiation. Here, we found that MyoD-induced transdifferentiation of fibroblasts to myoblasts promotes the expression of the ADP-ribosyltransferase ARTD1. Comprehensive analysis of the genome architecture by Hi-C and RNA-seq analysis during transdifferentiation indicated that ARTD1 locally contributed to A/B compartmentalization and coregulated a subset of MyoD target genes that were however not sufficient to alter transdifferentiation. Surprisingly, the expression of ARTD1 was accompanied by the continuous synthesis of nuclear ADP ribosylation that was neither dependent on the cell cycle nor induced by DNA damage. Conversely to the H2O2-induced ADP-ribosylation, the MyoD-dependent ADP-ribosylation was not associated to chromatin but rather localized to the nucleoplasm. Together, these data describe a MyoD-induced nucleoplasmic ADP-ribosylation that is observed particularly during transdifferentiation and thus potentially expands the plethora of cellular processes associated with ADP-ribosylation.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
57 - Biological sciences in general
Keywords
Ciències biològiques
Biologia cel·lular
Biologia del desenvolupament
Biologia molecular
Ciencias Biologicas
Biología del desarrollo
Biología molecular
Biología celular
Biological sciences
Cell biology
Developmental biology
Molecular biology
Pages
35
Publisher
Elsevier
Collection
24; 5
Is part of
iScience
Citation
Bisceglie, Lavinia; Hopp, Ann-Katrin; Gunasekera, Kapila [et al.]. MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation. iScience, 2021, 24(5), 102432. Disponible en: <https://www.sciencedirect.com/science/article/pii/S2589004221004004>. Fecha de acceso: 13 dic. 2021. DOI: 10.1016/j.isci.2021.102432
Grant agreement number
info:eu-repo/grantAgreement/EC/FP7/609989
info:eu-repo/grantAgreement/ES/1PE/SAF2016-75006-P
This item appears in the following Collection(s)
- Ciències de la Salut [745]
Rights
© 2021 The Author(s).This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/