MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation
Autor/a
Bisceglie, Lavinia
Hopp, Ann-Katrin
Gunasekera, Kapila
Wright, Roni H.G.
Le Dily, François
Vidal, Enrique
Dall'Agnese, Alessandra
Caputo, Luca
Nicoletti, Chiara
Puri, Pier Lorenzo
Beato, Miguel
Hottiger, Michael O.
Fecha de publicación
2021ISSN
2589-0042
Resumen
While protein ADP-ribosylation was reported to regulate differentiation and dedifferentiation, it has so far not been studied during transdifferentiation. Here, we found that MyoD-induced transdifferentiation of fibroblasts to myoblasts promotes the expression of the ADP-ribosyltransferase ARTD1. Comprehensive analysis of the genome architecture by Hi-C and RNA-seq analysis during transdifferentiation indicated that ARTD1 locally contributed to A/B compartmentalization and coregulated a subset of MyoD target genes that were however not sufficient to alter transdifferentiation. Surprisingly, the expression of ARTD1 was accompanied by the continuous synthesis of nuclear ADP ribosylation that was neither dependent on the cell cycle nor induced by DNA damage. Conversely to the H2O2-induced ADP-ribosylation, the MyoD-dependent ADP-ribosylation was not associated to chromatin but rather localized to the nucleoplasm. Together, these data describe a MyoD-induced nucleoplasmic ADP-ribosylation that is observed particularly during transdifferentiation and thus potentially expands the plethora of cellular processes associated with ADP-ribosylation.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
57 - Biología
Palabras clave
Ciències biològiques
Biologia cel·lular
Biologia del desenvolupament
Biologia molecular
Ciencias Biologicas
Biología del desarrollo
Biología molecular
Biología celular
Biological sciences
Cell biology
Developmental biology
Molecular biology
Páginas
35
Publicado por
Elsevier
Colección
24;5
Publicado en
iScience
Citación
Bisceglie, Lavinia; Hopp, Ann-Katrin; Gunasekera, Kapila [et al.]. MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation. iScience, 2021, 24(5), 102432. Disponible en: <https://www.sciencedirect.com/science/article/pii/S2589004221004004>. Fecha de acceso: 13 dic. 2021. DOI: 10.1016/j.isci.2021.102432
Número del acuerdo de la subvención
info:eu-repo/grantAgreement/EC/FP7/609989
info:eu-repo/grantAgreement/ES/1PE/SAF2016-75006-P
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Derechos
© 2021 The Author(s).This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Excepto si se señala otra cosa, la licencia del ítem se describe como https://creativecommons.org/licenses/by-nc-nd/4.0/