Polyphosphate degradation by Nudt3-Zn2+ mediates oxidative stress response
Autor/a
Samper Martín, Bàrbara
Sarrias, Ana
Lázaro, Blanca
Pérez-Montero, Marta
Rodríguez-Rodríguez, Rosalía
P.C. Ribeiro, Mariana
Bañón, Aitor
Wolfgeher, Donald
Jessen, Henning J.
Alsina, Berta
Clotet Erra, Josep
Kron, Stephen J.
Saiardi, Adolfo
Jiménez, Javier
Data de publicació
2021ISSN
2211-1247
Resum
Polyphosphate (polyP) is a polymer of hundreds of phosphate residues present in all organisms. In mammals, polyP is involved in crucial physiological processes, including coagulation, inflammation, and stress response. However, after decades of research, the metabolic enzymes are still unknown. Here, we purify and identify Nudt3, a NUDIX family member, as the enzyme responsible for polyP phosphatase activity in mammalian cells. We show that Nudt3 shifts its substrate specificity depending on the cation; specifically, Nudt3 is active on polyP when Zn2+ is present. Nudt3 has in vivo polyP phosphatase activity in human cells, and importantly, we show that cells with altered polyP levels by modifying Nudt3 protein amount present reduced viability upon oxidative stress and increased DNA damage, suggesting that polyP and Nudt3 play a role in oxidative stress protection. Finally, we show that Nudt3 is involved in the early stages of embryo development in zebrafish.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
57 - Biologia
61 - Medicina
Paraules clau
Polifosfat
Polifosfats de mamífers
Dany a l'ADN
Estrès oxidatiu
Polifosfato
Polifosfatos de mamíferos
Daño en el ADN
Estrés oxidativo
Polyphosphate
Mammalian polyphosphates
DNA damage
Oxidative stress
Pàgines
19
Publicat per
Elsevier
Col·lecció
37; 7
Publicat a
Cell Reports
Citació
Samper-Martín, Bàrbara; Sarrias, Ana; Lázaro, Blanca [et al.]. Polyphosphate degradation by Nudt3-Zn2+ mediates oxidative stress response. Cell Reports, 2021, 37(7), 110004. Disponible en: <https://www.sciencedirect.com/science/article/pii/S2211124721014820#bib30>. Fecha de acceso: 10 dic. 2021. DOI: 10.1016/j.celrep.2021.110004.
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- Ciències de la Salut [740]
Drets
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