Browsing Ciències Bàsiques by Author "138d4596-9bd7-4797-8741-74d4d5954783"
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Engineering a genome reduced bacterium to eliminate Staphylococcus aureus biofilms in vivo
Garrido, Victoria; Piñero Lambea, Carlos; Rodriguez Arce, Irene; Paetzold, Bernhard; Ferrar, Tony; Weber, Marc; Garcia Ramallo, Eva; Gallo López, Carolina; Collantes, María; Penuelas, Ivan; Serrano, Luis; Grillo, Maria-Jesus; Lluch, Maria (Molecular Systems Biology, 2021)Bacteria present a promising delivery system for treating human diseases. Here, we engineered the genome-reduced human lung pathogen Mycoplasma pneumoniae as a live biotherapeutic to treat biofilm-associated ... -
Engineering a genome-reduced bacterium to eliminate Staphylococcus aureus biofilms in vivo
Garrido, Victoria; Piñero Lambea, Carlos; Rodriguez Arce, Irene; Paetzold, Bernhard; Ferrar, Tony; Weber, Marc; Garcia-Ramallo Pla, EVA; Gallo, Carolina; Collantes, María; Peñuelas, Ivan; Serrano, Luis; Grillo, Maria-Jesus; Lluch, Maria (Molecular Systems Biology, 2021)Bacteria present a promising delivery system for treating human diseases. Here, we engineered the genome-reduced human lung pathogen Mycoplasma pneumoniae as a live biotherapeutic to treat biofilm-associated ... -
LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions
Shaw, Daniel; Miravet-Verde, Samuel; Piñero Lambea, Carlos; Serrano, Luis; Lluch, Maria (Microbial Biotechnology, 2021)The removal of unwanted genetic material is a key aspect in many synthetic biology efforts and often requires preliminary knowledge of which genomic regions are dispensable. Typically, these efforts are ... -
SURE editing: combining oligo-recombineering and programmable insertion/deletion of selection markers to efficiently edit the Mycoplasma pneumoniae genome
Piñero Lambea, Carlos; Eva, Garcia-Ramallo; Miravet-Verde, Samuel; Burgos, Raul; Scarpa, Margherita; Serrano, Luis; Lluch-Senar, Maria (Nucleic Acids Research, 2022)The development of advanced genetic tools is boosting microbial engineering which can potentially tackle wide-ranging challenges currently faced by our society. Here we present SURE editing, a ...