Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration
Author
Sawadkar, Prasad
Mandakhbayar, Nandin
Patel, Kapil D.
Olmas Buitrago, Jennifer
Hyun Kim, Tae
Rajasekar, Poojitha
Lali, Ferdinand
Kyriakidis, Christos
Rahmani, Benyamin
Mohanakrishnan, Jeviya
Dua, Rishbha
Greco, Karin
Lee, Jung-Hwan
Kim, Hae-Won
Knowles, Jonathan C.
García-Gareta, Elena
Publication date
2021ISSN
2041-7314
Abstract
Current gold standard to treat soft tissue injuries caused by trauma and pathological condition are autografts and off the shelf fillers, but they have inherent weaknesses like donor site morbidity, immuno-compatibility and graft failure. To overcome these limitations, tissue-engineered polymers are seeded with stem cells to improve the potential to restore tissue function. However, their interaction with native tissue is poorly understood so far. To study these interactions and improve outcomes, we have fabricated scaffolds from natural polymers (collagen, fibrin and elastin) by custom-designed processes and their material properties such as surface morphology, swelling, wettability and chemical cross-linking ability were characterised. By using 3D scaffolds, we comprehensive assessed survival, proliferation and phenotype of adipose-derived stem cells in vitro. In vivo, scaffolds were seeded with adipose-derived stem cells and implanted in a rodent model, with X-ray microtomography, histology and immunohistochemistry as read-outs. Collagenbased materials showed higher cell adhesion and proliferation in vitro as well as higher adipogenic properties in vivo. In contrast, fibrin demonstrated poor cellular and adipogenesis properties but higher angiogenesis. Elastin formed the most porous scaffold, with cells displaying a non-aggregated morphology in vitro while in vivo elastin was the most degraded scaffold. These findings of how polymers present in the natural polymers mimicking ECM and seeded with stem cells affect adipogenesis in vitro and in vivo can open avenues to design 3D grafts for soft tissue repair.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Col·lagen
Materials biomèdics
Teixits
Adipogènesi
Polímers naturals
Regeneració de teixits
Enginyeria de teixits
Colágeno
Materiales biomédicos
Tejidos
Adipogénesis
Polímeros naturales
Regeneración de tejidos
Ingeniería de tejidos
Collagen
Biomedical materials
Tissues
Adipogenesis
Natural polymers
Tissue regeneration
Tissue engineering
Pages
17
Publisher
Sage Journals
Collection
12;
Is part of
Journal of Tissue Engineering
Citation
Sawadkar, Prasad; Mandakhbayar, Nandin; Patel, Kapil D. [et al.]. Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration. Journal of Tissue Engineering, 2021, 12, p. 1-17. Disponible en: <https://journals.sagepub.com/doi/full/10.1177/20417314211019238>. Fecha de acceso: 2 jun. 2021. DOI: 10.1177/20417314211019238
This item appears in the following Collection(s)
- Ciències de la Salut [740]
Rights
Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc/4.0/