Detection and expression analysis of tet(B) in streptococcus oralis
Author
Àlvarez, Gerard
Nart Molina, José
Mor Reinoso, Carolina
Blanc, Vanessa
León, Rubén
Publication date
2019ISSN
2000-2297
Abstract
Tetracycline resistance can be achieved through tet genes, which code for efflux pumps, ribosomal protection proteins and inactivation enzymes. Some of these genes have only been described in either Gram-positive or Gram-negative bacteria. This is the case of tet(B), which codes for an efflux pump and, so far, had only been found in Gram-negative bacteria. In this study, tet(B) was detected in two clinical Streptococcus oralis strains isolated from the gingival sulci of two subjects. In both cases, the gene was completely sequenced, yielding 100% shared identity and coverage with other previously published sequences of tet(B). Moreover, we studied the expression of tet(B) using RT-qPCR in the isolates grown with and without tetracycline, detecting constitutive expression in only one of the isolates, with no signs of expression in the other one. This is the first time that the presence and expression of the tet(B) gene has been confirmed in Gram-positive bacteria, which highlights the potential of the genus Streptococcus to become a reservoir and a disseminator of antibiotic resistance genes in an environment so prone to horizontal gene transfer as is the oral biofilm.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Tetraciclines
Proteïnes
Enzims
Bacteris
Antibiòtics
Resistència als medicaments
Estreptococs
Tetraciclinas
Proteínas
Enzimas
Bacterias
Antibióticos
Resistencia a los medicamentos
Streptococcus
Tetracyclines
Proteins
Enzymes
Bacteria
Antibiotics
Drug resistance
Streptococci
Pages
8
Publisher
Taylor & Francis
Collection
11; 1
Is part of
Journal of Oral Microbiology
Citation
Arredondo, Alexandre; Àlvarez, Gerard; Nart Molina, José [et al.]. Detection and expression analysis of tet(B) in streptococcus oralis. Journal of Oral Microbiology, 2019, 11(1), 1643204. Disponible en: <https://www.tandfonline.com/doi/full/10.1080/20002297.2019.1643204>. Fecha de acceso: 21 may. 2021. DOI: 10.1080/20002297.2019.1643204
This item appears in the following Collection(s)
- Ciències de la Salut [740]
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