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dc.contributor.authorBuesa, Carlos
dc.contributor.authorMartínez-Gonzalez, José
dc.contributor.authorCasals i Farré, Núria
dc.contributor.authorHaro, Diego
dc.contributor.authorPiulachs, Maria-Dolors
dc.contributor.authorBellés, Xavier
dc.contributor.authorHegardt, Fausto G.
dc.date.accessioned2021-05-04T15:12:13Z
dc.date.available2021-05-04T15:12:13Z
dc.date.issued1994-04-22
dc.identifier.citationBuesa, Carlos; Martínez-Gonzalez, José; Casals Farré, Núria [et al.]. Blattella germanica has two HMG-CoA synthase genes. Both are regulated in the ovary during the gonadotrophic cycle. Journal of Biological Chemistry, 1994, 289(16), p. 11707-11713. Disponible en: <https://www.sciencedirect.com/science/article/pii/S0021925817326303>. Fecha de acceso: 4 may. 2021. DOI: 10.1016/S0021-9258(17)32630-3ca
dc.identifier.issn0021-9258ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/2517
dc.description.abstractThe isoprenoid pathway leads to various essential non-sterol products in insects. These end products have a crucial role in growth, differentiation, sexual maturation, and reproduction. 3-Hydroxy-3-methylglutaryl co-enzyme A (HMG-CoA) synthase (EC 4.1.3.5.) has generally been considered one of the committed steps of the pathway. We had previously reported the cloning of a cytosolic HMG-CoA synthase cDNA in Blattella germanica; we have now isolated and characterized a new cDNA clone for HMG-CoA synthase in this insect. Analysis of this 1716-base pair cDNA reveals a deduced protein of 455 residues with a molecular mass of 51,424 Da. The two HMG-CoA synthases have 69% identical amino acid residues, and both lack an N-terminal leader peptide to target the protein into mitochondria. This HMG-CoA synthase cDNA can revert the Chinese hamster ovary-K1-derived cell line, Mev-1, which is a defective mutant for HMG-CoA synthase. Both HMG-CoA synthase genes are expressed differently throughout development. Analysis of adult tissues shows higher expression in ovary and fat body. The expression of HMG-CoA synthase (EC 4.1.3.5.) and reductase (EC 1.1.1.34) genes during the gonadotrophic cycle in B. germanica shows that the three genes of the isoprenoid pathway are developmentally regulated in the ovary.en
dc.format.extent7ca
dc.language.isoengca
dc.publisherAmerican Society for Biochemistry and Molecular Biologyca
dc.relation.ispartofJournal of Biological Chemistryca
dc.relation.ispartofseries269;16
dc.rightsUnder a Creative Commons license. This is an Open Access article under the CC BY license.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.otherGinecologiaca
dc.subject.otherAparell genital femení -- Malaltiesca
dc.subject.otherOvaris -- Malaltiesca
dc.subject.otherGinecologíaes
dc.subject.otherVagina -- Enfermedadeses
dc.subject.otherOvarios -- Enfermedadeses
dc.subject.otherGynecologyen
dc.subject.otherFemale genitalia -- Diseasesen
dc.subject.otherOvaries -- Diseasesen
dc.titleBlattella germanica has two HMG-CoA synthase genes. Both are regulated in the ovary during the gonadotrophic cycleen
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc61ca
dc.identifier.doihttps://dx.doi.org/10.1016/S0021-9258(17)32630-3ca


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Under a Creative Commons license. This is an Open Access article under the CC BY license.
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/