Blattella germanica has two HMG-CoA synthase genes. Both are regulated in the ovary during the gonadotrophic cycle
Autor/a
Buesa, Carlos
Martínez-Gonzalez, José
Casals i Farré, Núria
Haro, Diego
Piulachs, Maria-Dolors
Bellés, Xavier
Hegardt, Fausto G.
Data de publicació
1994-04-22ISSN
0021-9258
Resum
The isoprenoid pathway leads to various essential non-sterol products in insects. These end products have a crucial role in growth, differentiation, sexual maturation, and reproduction. 3-Hydroxy-3-methylglutaryl co-enzyme A (HMG-CoA) synthase (EC 4.1.3.5.) has generally been considered one of the committed steps of the pathway. We had previously reported the cloning of a cytosolic HMG-CoA synthase cDNA in Blattella germanica; we have now isolated and characterized a new cDNA clone for HMG-CoA synthase in this insect. Analysis of this 1716-base pair cDNA reveals a deduced protein of 455 residues with a molecular mass of 51,424 Da. The two HMG-CoA synthases have 69% identical amino acid residues, and both lack an N-terminal leader peptide to target the protein into mitochondria. This HMG-CoA synthase cDNA can revert the Chinese hamster ovary-K1-derived cell line, Mev-1, which is a defective mutant for HMG-CoA synthase. Both HMG-CoA synthase genes are expressed differently throughout development. Analysis of adult tissues shows higher expression in ovary and fat body. The expression of HMG-CoA synthase (EC 4.1.3.5.) and reductase (EC 1.1.1.34) genes during the gonadotrophic cycle in B. germanica shows that the three genes of the isoprenoid pathway are developmentally regulated in the ovary.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
61 - Medicina
Paraules clau
Ginecologia
Aparell genital femení -- Malalties
Ovaris -- Malalties
Ginecología
Vagina -- Enfermedades
Ovarios -- Enfermedades
Gynecology
Female genitalia -- Diseases
Ovaries -- Diseases
Pàgines
7
Publicat per
American Society for Biochemistry and Molecular Biology
Col·lecció
269; 16
Publicat a
Journal of Biological Chemistry
Citació
Buesa, Carlos; Martínez-Gonzalez, José; Casals Farré, Núria [et al.]. Blattella germanica has two HMG-CoA synthase genes. Both are regulated in the ovary during the gonadotrophic cycle. Journal of Biological Chemistry, 1994, 289(16), p. 11707-11713. Disponible en: <https://www.sciencedirect.com/science/article/pii/S0021925817326303>. Fecha de acceso: 4 may. 2021. DOI: 10.1016/S0021-9258(17)32630-3
Aquest element apareix en la col·lecció o col·leccions següent(s)
- Ciències de la Salut [740]
Drets
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