Biofunctionalization with a TGFβ-1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in beagle dogs
Author
Cirera, Andrea
Manzanares-Céspedes, Maria Cristina
Sevilla Sánchez, Pablo
Ortiz-Hernández, Monica
Galindo-Moreno, Pablo
Gil Mur, Francisco Javier
Publication date
2019-09-27ISSN
1996-1944
Abstract
Objectives: The aim of this research was to determine the osseointegration of two presentations of biphasic calcium phosphate (BCP) biomaterial—one untreated and another submitted to biofunctionalization with a TGF-β1 inhibitor peptide, P144, on dental alveolus. Materials and Methods: A synthetic bone graft was used, namely, (i) Maxresorb® (Botiss Klockner) (n = 12), and (ii) Maxresorb® (Botiss Klockner) biofunctionalized with P144 peptide (n = 12). Both bone grafts were implanted in the two hemimandibles of six beagle dogs in the same surgical time, immediately after tooth extraction. Two dogs were sacrificed 2, 4, and 8 weeks post implant insertion, respectively. The samples were submitted to histomorphometrical and histological analyses. For each sample, we quantified the new bone growth and the new bone formed around the biomaterial’s granules. After optical microscopic histological evaluation, selected samples were studied using backscattered scanning electron microscopy (BS-SEM). Results: The biofunctionalization of the biomaterial’s granules maintains a stable membranous bone formation throughout the experiment timeline, benefitting from the constant presence of vascular structures in the alveolar space, in a more active manner that in the control samples. Better results in the experimental groups were proven both by quantitative and qualitative analysis. Conclusions: Synthetic bone graft biofunctionalization results in slightly better quantitative parameters of the implant’s osseointegration. The qualitative histological and ultramicroscopic analysis shows that biofunctionalization may shorten the healing period of dental biomaterials. View Full-Text
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Gossos
Ossos -- Cirurgia
Pròtesis
Perros
Huesos -- Cirugía
Prótesis
Dogs
Bones--Surgery
Prosthesis
Pages
23
Publisher
MDPI
Collection
12; 19
Is part of
Materials
Citation
Cirera, Andrea; Manzanares, Maria Cristina; Sevilla, Pablo [et al.]. Biofunctionalization with a TGFβ-1 inhibitor peptide in the osseointegration of synthetic bone grafts: an in vivo study in beagle dogs. Materials, 2019, vol. 12, núm. 19, 3168. Disponible en: <https://www.mdpi.com/1996-1944/12/19/3168>. Fecha de acceso: 8 feb. 2020. DOI: 10.3390/ma12193168
Grant agreement number
info:eu-repo/grantAgreement/ES/2PE/RTI2018-098075-B-C22
This item appears in the following Collection(s)
- Ciències de la Salut [740]
Rights
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/