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dc.contributor.authorDeniz, Özgen
dc.contributor.authorFlores, Óscar
dc.contributor.authorAldea Malo, Martí
dc.contributor.authorSoler-López, Montserrat
dc.contributor.authorOrozco, Modesto
dc.date.accessioned2019-10-22T10:17:27Z
dc.date.available2019-10-22T10:17:27Z
dc.date.issued2016-01-28
dc.identifier.citationDeniz, Özgen; Flores, Óscar; Aldea Malo, Martí; Soler-López, Montserrat; Orozco, Modesto. «Nucleosome architecture throughout the cell cycle». Scientific Reports, 2016, vol. 6, art. 19729. Disponible en: <https://www.nature.com/articles/srep19729>. Fecha de acceso: 22 oct. 2019. DOI: 10.1038/srep19729ca
dc.identifier.issn2045-2322ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/1267
dc.description.abstractNucleosomes provide additional regulatory mechanisms to transcription and DNA replication by mediating the access of proteins to DNA. During the cell cycle chromatin undergoes several conformational changes, however the functional significance of these changes to cellular processes are largely unexplored. Here, we present the first comprehensive genome-wide study of nucleosome plasticity at single base-pair resolution along the cell cycle in Saccharomyces cerevisiae. We determined nucleosome organization with a specific focus on two regulatory regions: transcription start sites (TSSs) and replication origins (ORIs). During the cell cycle, nucleosomes around TSSs display rearrangements in a cyclic manner. In contrast to gap (G1 and G2) phases, nucleosomes have a fuzzier organization during S and M phases, Moreover, the choreography of nucleosome rearrangements correlate with changes in gene expression during the cell cycle, indicating a strong association between nucleosomes and cell cycle-dependent gene functionality. On the other hand, nucleosomes are more dynamic around ORIs along the cell cycle, albeit with tighter regulation in early firing origins, implying the functional role of nucleosomes on replication origins. Our study provides a dynamic picture of nucleosome organization throughout the cell cycle and highlights the subsequent impact on transcription and replication activity.ca
dc.format.extent11ca
dc.language.isoengca
dc.publisherNature Researchca
dc.relation.ispartofScientific Reportsca
dc.relation.ispartofseries6;
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/ca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.otherADNca
dc.subject.otherDNAca
dc.subject.otherCèl·lulesca
dc.subject.otherCellsca
dc.subject.otherCélulasca
dc.titleNucleosome architecture throughout the cell cycleca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.embargo.termscapca
dc.subject.udc61ca
dc.identifier.doihttps://doi.org/10.1038/srep19729ca


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