Nucleosome architecture throughout the cell cycle
Autor/a
Deniz, Özgen
Flores, Óscar
Aldea Malo, Martí
Soler-López, Montserrat
Orozco, Modesto
Data de publicació
2016-01-28ISSN
2045-2322
Resum
Nucleosomes provide additional regulatory mechanisms to transcription and DNA replication
by mediating the access of proteins to DNA. During the cell cycle chromatin undergoes several
conformational changes, however the functional significance of these changes to cellular processes
are largely unexplored. Here, we present the first comprehensive genome-wide study of nucleosome
plasticity at single base-pair resolution along the cell cycle in Saccharomyces cerevisiae. We determined
nucleosome organization with a specific focus on two regulatory regions: transcription start sites (TSSs)
and replication origins (ORIs). During the cell cycle, nucleosomes around TSSs display rearrangements
in a cyclic manner. In contrast to gap (G1 and G2) phases, nucleosomes have a fuzzier organization
during S and M phases, Moreover, the choreography of nucleosome rearrangements correlate with
changes in gene expression during the cell cycle, indicating a strong association between nucleosomes
and cell cycle-dependent gene functionality. On the other hand, nucleosomes are more dynamic around
ORIs along the cell cycle, albeit with tighter regulation in early firing origins, implying the functional
role of nucleosomes on replication origins. Our study provides a dynamic picture of nucleosome
organization throughout the cell cycle and highlights the subsequent impact on transcription and
replication activity.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
Anglès
Matèries (CDU)
61 - Medicina
Paraules clau
ADN
DNA
Cèl·lules
Cells
Células
Pàgines
11
Publicat per
Nature Research
Col·lecció
6;
Publicat a
Scientific Reports
Citació
Deniz, Özgen; Flores, Óscar; Aldea Malo, Martí; Soler-López, Montserrat; Orozco, Modesto. «Nucleosome architecture throughout the cell cycle». Scientific Reports, 2016, vol. 6, art. 19729. Disponible en: <https://www.nature.com/articles/srep19729>. Fecha de acceso: 22 oct. 2019. DOI: 10.1038/srep19729
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