Tissue-engineered mucosa is a suitable model to quantify the acute biological effects of ionizing radiation
Fecha de publicación
2025ISSN
0901-5027
Resumen
The aim of this study was to evaluate the suitability of tissue-engineered mucosa (TEM) as a model for studying the acute effects of ionizing radiation (IR) on the oral mucosa. TEM and native non-keratinizing oral mucosa (NNOM) were exposed to a single dose of 16.5 Gy and harvested at 1, 6, 24, 48, and 72 h post-irradiation. DNA damage induced by IR was determined using p53 binding protein 1 (53BP1), and DNA repair was determined using Rad51. Various components of the epithelial layer, basement membrane, and underlying connective tissue were analyzed using immunohistochemistry. The expression of cytokines interleukin-1β (IL-1β) and transforming growth factor beta 1 (TGF-β1) was analyzed using an enzyme-linked immunosorbent assay. The expression of DNA damage protein 53BP1 and repair protein Rad51 were increased post-irradiation. The expression of keratin 19, vimentin, collage type IV, desmoglein 3, and integrins α6 and β4 was altered post-irradiation. Proliferation significantly decreased at 24, 48, and 72 h post-irradiation in both NNOM and TEM. IR increased the secretion of IL-1β, whereas TGF-β1 secretion was not altered. All observed IR-induced alterations in TEM were also observed in NNOM. Based on the similar response of TEM and NNOM to IR we consider our TEM construct a suitable model to quantify the acute biological effects of IR.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
57 - Biología
Palabras clave
Páginas
9
Publicado por
Elsevier
Colección
42; 8
Publicado en
International Journal of Oral and Maxillofacial Surgery
Citación
Tra, W. M. W.; Tuk, B.: Van Neck, J. W. [et al.]. Tissue-engineered mucosa is a suitable model to quantify the acute biological effects of ionizing radiation. International Journal of Oral and Maxillofacial Surgery, 2013, 42(8), p. 939-948. Disponible en: <https://www.sciencedirect.com/science/article/pii/S0901502713001161?via%3Dihub>. Fecha de acceso: 8 jul. 2025. DOI: 10.1016/j.ijom.2013.01.025
Este ítem aparece en la(s) siguiente(s) colección(ones)
- Ciències de la Salut [952]
Derechos
© 2013 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights