dc.contributor.author | Peyman, Mona | |
dc.contributor.author | Babin-Ebell, Anna | |
dc.contributor.author | Rodríguez Rodríguez, Rosalía | |
dc.contributor.author | Rigon, Matilde | |
dc.contributor.author | Aguilar-Recarte, David | |
dc.contributor.author | Villarroya, Joan | |
dc.contributor.author | Planavila, Anna | |
dc.contributor.author | Villarroya, Francesc | |
dc.contributor.author | Palomer, Xavier | |
dc.contributor.author | Barroso, Emma | |
dc.contributor.author | Vázquez-Carrera, Manuel | |
dc.date.accessioned | 2024-07-11T10:52:16Z | |
dc.date.available | 2024-07-11T10:52:16Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Peyman, Mona; Babin-Ebell, Anna; Rodríguez Rodríguez, Rosalía [et al.]. SIRT1 regulates hepatic vldlr levels. Cell Communication and Signaling, 2024, 22, 297. Disponible en: <https://biosignaling.biomedcentral.com/articles/10.1186/s12964-024-01666-y>. Fecha de acceso: 11 jul. 2024. DOI: 10.1186/s12964-024-01666-y | ca |
dc.identifier.issn | 1478-811X | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/4307 | |
dc.description.abstract | Background: Endoplasmic reticulum (ER) stress-mediated increases in the hepatic levels of the very low-density lipoprotein (VLDL) receptor (VLDLR) promote hepatic steatosis by increasing the delivery of triglyceride-rich lipoproteins to the liver. Here, we examined whether the NAD(+)-dependent deacetylase sirtuin 1 (SIRT1) regulates hepatic lipid accumulation by modulating VLDLR levels and the subsequent uptake of triglyceride-rich lipoproteins. Methods: Rats fed with fructose in drinking water, Sirt1−/− mice, mice treated with the ER stressor tunicamycin with or without a SIRT1 activator, and human Huh-7 hepatoma cells transfected with siRNA or exposed to tunicamycin or different inhibitors were used. Results: Hepatic SIRT1 protein levels were reduced, while those of VLDLR were upregulated in the rat model of metabolic dysfunction-associated steatotic liver disease (MASLD) induced by fructose-drinking water. Moreover, Sirt1−/− mice displayed increased hepatic VLDLR levels that were not associated with ER stress, but were accompanied by an increased expression of hypoxia-inducible factor 1α (HIF-1α)-target genes. The pharmacological inhibition or gene knockdown of SIRT1 upregulated VLDLR protein levels in the human Huh-7 hepatoma cell line, with this increase abolished by the pharmacological inhibition of HIF-1α. Finally, SIRT1 activation prevented the increase in hepatic VLDLR protein levels in mice treated with the ER stressor tunicamycin. Conclusions: Overall, these findings suggest that SIRT1 attenuates fatty liver development by modulating hepatic VLDLR levels. | ca |
dc.format.extent | 11 | ca |
dc.language.iso | eng | ca |
dc.publisher | Springer Nature | ca |
dc.relation.ispartof | Cell Communication and Signaling | ca |
dc.relation.ispartofseries | 22 | |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. | ca |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject.other | MASLD | ca |
dc.subject.other | SIRT1 | ca |
dc.subject.other | VLDLR | ca |
dc.subject.other | HIF-1α | ca |
dc.subject.other | ER stress | ca |
dc.title | SIRT1 regulates hepatic vldlr levels | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 61 | ca |
dc.identifier.doi | https://dx.doi.org/10.1186/s12964-024-01666-y | ca |