dc.contributor.author | Quiles, M Teresa | |
dc.contributor.author | Rodríguez-Contreras, Alejandra | |
dc.contributor.author | Guillem-Marti, Jordi | |
dc.contributor.author | Punset fuste, Miquel | |
dc.contributor.author | Sanchez-Soto, Miguel | |
dc.contributor.author | López-Cano, Manuel | |
dc.contributor.author | Sabadell, Jordi | |
dc.contributor.author | Velasco, Janice | |
dc.contributor.author | Armengol Carrasco, Manuel | |
dc.contributor.author | Manero, José María | |
dc.contributor.author | Arbós, Maria Antònia | |
dc.date.accessioned | 2024-04-08T08:09:03Z | |
dc.date.available | 2024-04-08T08:09:03Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Quiles, M Teresa; Rodríguez-Contreras, Alejandra; Guillem-Marti, Jordi [et al.]. Effect of functionalization of texturized polypropylene surface by silanization and HBII-RGD attachment on response of primary abdominal and vaginal fibroblasts. Polymers, 2024, 16(5), 667. Disponible en: <https://www.mdpi.com/2073-4360/16/5/667>. Fecha de acceso: 8 abr. 2024. DOI: 10.3390/polym16050667 | ca |
dc.identifier.issn | 2073-4360 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/4193 | |
dc.description.abstract | Soft tissue defects, such as incisional hernia or pelvic organ prolapse, are prevalent pathologies characterized by a tissue microenvironment rich in fragile and dysfunctional fibroblasts. Precision medicine could improve their surgical repair, currently based on polymeric materials. Nonetheless, biomaterial-triggered interventions need first a better understanding of the cell-material interfaces that truly consider the patients’ biology. Few tools are available to study the interactions between polymers and dysfunctional soft tissue cells in vitro. Here, we propose polypropylene (PP) as a matrix to create microscale surfaces w/wo functionalization with an HBII-RGD molecule, a fibronectin fragment modified to include an RGD sequence for promoting cell attachment and differentiation. Metal mold surfaces were roughened by shot blasting with aluminum oxide, and polypropylene plates were obtained by injection molding. HBII-RGD was covalently attached by silanization. As a proof of concept, primary abdominal and vaginal wall fasciae fibroblasts from control patients were grown on the new surfaces. Tissue-specific significant differences in cell morphology, early adhesion and cytoskeletal structure were observed. Roughness and biofunctionalization parameters exerted unique and combinatorial effects that need further investigation. We conclude that the proposed model is effective and provides a new framework to inform the design of smart materials for the treatment of clinically compromised tissues. | ca |
dc.format.extent | 24 | ca |
dc.language.iso | eng | ca |
dc.publisher | MDPI | ca |
dc.relation.ispartof | Polymers | ca |
dc.relation.ispartofseries | 16;5 | |
dc.rights | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | ca |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject.other | Polipropilè | ca |
dc.subject.other | Hèrnia incisional | ca |
dc.subject.other | Prolapse d'òrgans pèlvics | ca |
dc.subject.other | Fragment de fibronectina | ca |
dc.subject.other | Rugositat | ca |
dc.subject.other | Funcionalització | ca |
dc.subject.other | Fibroblast | ca |
dc.subject.other | Citoesquelet | ca |
dc.subject.other | Adhesió cel·lular | ca |
dc.subject.other | Nucli cel·lular | ca |
dc.subject.other | Malla quirúrgica | ca |
dc.subject.other | Polipropileno | ca |
dc.subject.other | Hernia incisional | ca |
dc.subject.other | Prolapso de órganos pélvicos | ca |
dc.subject.other | Fragmento de fibronectina | ca |
dc.subject.other | Aspereza | ca |
dc.subject.other | Funcionalización | ca |
dc.subject.other | Fibroblasto | ca |
dc.subject.other | Citoesqueleto | ca |
dc.subject.other | Adhesión celular | ca |
dc.subject.other | Nucleo celular | ca |
dc.subject.other | Malla quirurgica | ca |
dc.subject.other | Polypropylene | ca |
dc.subject.other | Incisional hernia | ca |
dc.subject.other | Pelvic organ prolapse | ca |
dc.subject.other | Fibronectin fragment | ca |
dc.subject.other | Roughness | ca |
dc.subject.other | Functionalization | ca |
dc.subject.other | Fibroblast | ca |
dc.subject.other | Cytoskeleton | ca |
dc.subject.other | Cell adhesion | ca |
dc.subject.other | Cell nucleus | ca |
dc.subject.other | Surgical mesh | ca |
dc.title | Effect of functionalization of texturized polypropylene surface by silanization and HBII-RGD attachment on response of primary abdominal and vaginal fibroblasts | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 61 | ca |
dc.identifier.doi | https://dx.doi.org/10.3390/polym16050667 | ca |