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Müller glia fused with adult stem cells undergo neural differentiation in human retinal models
dc.contributor.author | Bonilla-Pons, Sergi Àngel | |
dc.contributor.author | Nakagawa, Shoma | |
dc.contributor.author | Garreta Bahima, Elena | |
dc.contributor.author | Fernández-Blanco, Álvaro | |
dc.contributor.author | Pesaresi, Martina | |
dc.contributor.author | D'Antin, Justin Christopher | |
dc.contributor.author | Perez, Ruben Sebastian | |
dc.contributor.author | Greco, Daniela | |
dc.contributor.author | Domínguez-Sala, Eduardo | |
dc.contributor.author | Gómez-Riera, Raúl | |
dc.contributor.author | Barraquer Compte, Rafael Ignacio | |
dc.contributor.author | Dierssen, Mara | |
dc.contributor.author | Pulido, Nuria Montserrat | |
dc.contributor.author | Cosma, Maria Pia | |
dc.date.accessioned | 2024-02-14T15:20:20Z | |
dc.date.available | 2024-02-14T15:20:20Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Bonilla-Pons, Sergi Àngel; Nakagawa, Shoma; Garreta Bahima, Elena [et al.]. Müller glia fused with adult stem cells undergo neural differentiation in human retinal models. eBioMedicine, 2022, 77, 103914. Disponible en: <https://www.sciencedirect.com/science/article/pii/S2352396422000986?via%3Dihub>. Fecha de acceso: 14 feb. 2024. DOI: 10.1016/j.ebiom.2022.103914. | ca |
dc.identifier.issn | 2352-3964 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/4091 | |
dc.description.abstract | Background: Visual impairments are a critical medical hurdle to be addressed in modern society. Müller glia (MG) have regenerative potential in the retina in lower vertebrates, but not in mammals. However, in mice, in vivo cell fusion between MG and adult stem cells forms hybrids that can partially regenerate ablated neurons. Methods: We used organotypic cultures of human retina and preparations of dissociated cells to test the hypothesis that cell fusion between human MG and adult stem cells can induce neuronal regeneration in human systems. Moreover, we established a microinjection system for transplanting human retinal organoids to demonstrate hybrid differentiation. Findings: We first found that cell fusion occurs between MG and adult stem cells, in organotypic cultures of human retina as well as in cell cultures. Next, we showed that the resulting hybrids can differentiate and acquire a proto-neural electrophysiology profile when the Wnt/beta-catenin pathway is activated in the adult stem cells prior fusion. Finally, we demonstrated the engraftment and differentiation of these hybrids into human retinal organoids. Interpretation: We show fusion between human MG and adult stem cells, and demonstrate that the resulting hybrid cells can differentiate towards neural fate in human model systems. Our results suggest that cell fusion-mediated therapy is a potential regenerative approach for treating human retinal dystrophies. | ca |
dc.format.extent | 22 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | eBioMedicine | ca |
dc.relation.ispartofseries | 77 | |
dc.rights | © Under a Creative Commons license. | ca |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.other | Regeneració de la retina | ca |
dc.subject.other | Fusió cel·lular | ca |
dc.subject.other | Organoides | ca |
dc.subject.other | Cèl·lula mare | ca |
dc.subject.other | Diferenciació neuronal | ca |
dc.subject.other | Muller glia | ca |
dc.subject.other | Regeneración de retina | ca |
dc.subject.other | Fusión celular | ca |
dc.subject.other | Organoides | ca |
dc.subject.other | Célula madre | ca |
dc.subject.other | Diferenciación neuronal | ca |
dc.subject.other | Müller glía | ca |
dc.subject.other | Retina regeneration | ca |
dc.subject.other | Cell fusion | ca |
dc.subject.other | Organoids | ca |
dc.subject.other | Stem cell | ca |
dc.subject.other | Neural differentiation | ca |
dc.subject.other | Muller glia | ca |
dc.title | Müller glia fused with adult stem cells undergo neural differentiation in human retinal models | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 617 | ca |
dc.identifier.doi | https://dx.doi.org/10.1016/j.ebiom.2022.103914 | ca |
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