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Protocol to quantify polyphosphate in human cell lines using a tagged PPBD peptide

dc.contributor.authorJiménez, Javier
dc.contributor.authorLázaro, Blanca
dc.contributor.authorSarrias, Ana
dc.contributor.authorTadeo, Francisco J.
dc.contributor.authorPérez-Montero, Marta
dc.contributor.authorClotet, Josep
dc.contributor.authorBru Rullo, Samuel
dc.date.accessioned2024-01-23T16:10:12Z
dc.date.available2024-01-23T16:10:12Z
dc.date.issued2022
dc.identifier.citationJiménez, Javier; Lázaro, Blanca; Sarrias, Ana [et al.]. Protocol to quantify polyphosphate in human cell lines using a tagged PPBD peptide. STAR Protocols, 2022, 3(2), 101363. Disponible en: <https://www.sciencedirect.com/science/article/pii/S266616672200243X?via%3Dihub>. Fecha de acceso: 23 ene. 2024. DOI: 10.1016/j.xpro.2022.101363ca
dc.identifier.issn2666-1667ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/3953
dc.descriptionThis work was supported by and is part of the I+D+i grant ref. PGC2018-096597-B-I00 (to J.J. and J.C.) by the Spanish Ministerio de Ciencia e Innovación (MCIN). We want to thank our collaborators in this project: Adolfo Saiardi, Henning J. Jessen, Berta Alsina, and Stephen J. Kron.en
dc.description.abstractPolyphosphate (polyP) is an evolutionarily conserved polymer of phosphates that is difficult to study in human cells because of its low concentration and high lability. First, we described how to express and purify Xpress-tagged PPBD (Ppx1 PolyP Binding Domain). We describe the detection and quantification of nuclear polyP in HEK293T cells using Xpress-PPBD, Xpress antibody, and Alexa-conjugated secondary antibodies. We have also used this protocol in SH-SY5Y HeLa and HEK293 cells. For complete details on the use and execution of this protocol, please refer to Samper-Martín et al. (2021).ca
dc.format.extent10ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofSTAR Protocolsca
dc.relation.ispartofseries3;2
dc.rightsUnder a Creative Commons license.ca
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.otherBiologia cel·lularca
dc.subject.otherAnticòs de microscòpiaca
dc.subject.otherBioquímica de proteïnesca
dc.subject.otherExpressió i purificació de proteïnesca
dc.subject.otherBiología Celularca
dc.subject.otherAnticuerpo de microscopíaca
dc.subject.otherBioquímica de Proteínasca
dc.subject.otherExpresión y purificación de proteínasca
dc.subject.otherCell Biologyca
dc.subject.otherMicroscopy antibodyca
dc.subject.otherProtein biochemistryca
dc.subject.otherProtein expression and purificationca
dc.titleProtocol to quantify polyphosphate in human cell lines using a tagged PPBD peptideca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MICINN/PGC2018-096597-B-I00
dc.subject.udc57ca
dc.identifier.doihttps://dx.doi.org/10.1016/j.xpro.2022.101363ca


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