dc.contributor.author | Soler Vázquez, M. Carmen | |
dc.contributor.author | Romero, María del Mar | |
dc.contributor.author | Todorcevic, Marijana | |
dc.contributor.author | Delgado, Katia | |
dc.contributor.author | Calatayud, Carles | |
dc.contributor.author | Benitez Amaro, Aleyda | |
dc.contributor.author | La Chica Lhoëst, Maria Teresa | |
dc.contributor.author | Mera, Paula | |
dc.contributor.author | Zagmutt, Sebastián | |
dc.contributor.author | Bastías Pérez, Marianela | |
dc.contributor.author | Ibeas, Kevin | |
dc.contributor.author | Casals, Núria | |
dc.contributor.author | Escolà Gil, Joan Carles | |
dc.contributor.author | Llorente Cortés, Vicenta | |
dc.contributor.author | Consiglio, Antonella | |
dc.contributor.author | Serra, Dolors | |
dc.contributor.author | Herrero, Laura | |
dc.date.accessioned | 2023-05-02T13:43:20Z | |
dc.date.available | 2023-05-02T13:43:20Z | |
dc.date.issued | 2023-05 | |
dc.identifier.citation | Soler Vázquez, M. Carmen; Romero, María del Mar; Todorcevic, Marijana [et al.]. Implantation of CPT1AM-expressing adipocytes reduces obesity and glucose intolerance in mice. Metabolic Engineering, 2023, 77, 256-272. Disponible en: <https://www.sciencedirect.com/science/article/pii/S1096717623000630?via%3Dihub>. Fecha de acceso: 2 may. 2023. DOI: 10.1016/j.ymben.2023.04.010 | ca |
dc.identifier.issn | 1096-7176 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/3685 | |
dc.description.abstract | Obesity and its associated metabolic comorbidities are a rising global health and social issue, with novel therapeutic approaches urgently needed. Adipose tissue plays a key role in the regulation of energy balance and adipose tissue-derived mesenchymal stem cells (AT-MSCs) have gained great interest in cell therapy. Carnitine palmitoyltransferase 1A (CPT1A) is the gatekeeper enzyme for mitochondrial fatty acid oxidation. Here, we aimed to generate adipocytes expressing a constitutively active CPT1A form (CPT1AM) that can improve the obese phenotype in mice after their implantation. AT-MSCs were differentiated into mature adipocytes, subjected to lentivirus-mediated expression of CPT1AM or the GFP control, and subcutaneously implanted into mice fed a high-fat diet (HFD). CPT1AM-implanted mice showed lower body weight, hepatic steatosis and serum insulin and cholesterol levels alongside improved glucose tolerance. HFD-induced increases in adipose tissue hypertrophy, fibrosis, inflammation, endoplasmic reticulum stress and apoptosis were reduced in CPT1AM-implanted mice. In addition, the expression of mitochondrial respiratory chain complexes was enhanced in the adipose tissue of CPT1AM-implanted mice. Our results demonstrate that implantation of CPT1AM-expressing AT-MSC-derived adipocytes into HFD-fed mice improves the obese metabolic phenotype, supporting the future clinical use of this ex vivo gene therapy approach. | en |
dc.format.extent | 17 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | Metabolic Engineering | ca |
dc.relation.ispartofseries | 77 | |
dc.relation.uri | https://www.sciencedirect.com/science/article/pii/S1096717623000630?via%3Dihub | ca |
dc.rights | Under a Creative Commons license. | ca |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.other | Obesitat | ca |
dc.subject.other | Diabetis tipus 2 | ca |
dc.subject.other | Teixit adipós | ca |
dc.subject.other | Cèl·lules mare mesenquimals derivades del teixit adipós | ca |
dc.subject.other | Carnitina palmitoiltransferasa 1A | ca |
dc.subject.other | Obesidad | es |
dc.subject.other | Diabetes tipo 2 | es |
dc.subject.other | Tejido adiposo | es |
dc.subject.other | Células madre mesenquimales derivadas de tejido adiposo | es |
dc.subject.other | Carnitina palmitoiltransferasa 1A | es |
dc.subject.other | Obesity | en |
dc.subject.other | Type 2 diabetes | en |
dc.subject.other | Adipose tissue | en |
dc.subject.other | Adipose tissue-derived mesenchymal stem cells | en |
dc.subject.other | Carnitine palmitoyltransferase 1A | en |
dc.title | Implantation of CPT1AM-expressing adipocytes reduces obesity and glucose intolerance in mice | en |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 61 | ca |
dc.identifier.doi | https://dx.doi.org/10.1016/j.ymben.2023.04.010 | ca |