Functional signatures of ex-vivo dental caries onset
Author
Sharma, Ashok K.
Witthuhn, Bruce
Rudney, Joel D.
Publication date
2022ISSN
2000-2297
Abstract
Background: The etiology of dental caries remains poorly understood. With the advent of next-generation sequencing, a number of studies have focused on the microbial ecology of the disease. However, taxonomic associations with caries have not been consistent. Researchers have also pursued function-centric studies of the caries microbial communities aiming to identify consistently conserved functional pathways. A major question is whether changes in microbiome are a cause or a consequence of the disease. Thus, there is a critical need to define conserved functional signatures at the onset of dental caries. Methods: Since it is unethical to induce carious lesions clinically, we developed an innovative longitudinal ex-vivo model integrated with the advanced non-invasive multiphoton second harmonic generation bioimaging to spot the very early signs of dental caries, combined with 16S rRNA short amplicon sequencing and liquid chromatography-mass spectrometry-based targeted metabolomics. Findings: For the first time, we induced longitudinally monitored caries lesions validated with the scanning electron microscope. Consequently, we spotted the caries onset and, associated with it, distinguished five differentiating metabolites – Lactate, Pyruvate, Dihydroxyacetone phosphate, Glyceraldehyde 3-phosphate (upregulated) and Fumarate (downregulated). Those metabolites co-occurred with certain bacterial taxa; Streptococcus, Veillonella, Actinomyces, Porphyromonas, Fusobacterium, and Granulicatella, regardless of the abundance of other taxa.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Càries dental
Model longitudinal
Signatures
Metabolòmica
Genòmica
Bioimatge no invasiva
Caries dental
Modelo longitudinal
Firmas
Metabolómica
Genómica
Bioimagen no invasiva
Dental caries
Longitudinal model
Signatures
Metabolomics
Genomics
Non-invasive bioimaging
Pages
20
Publisher
Taylor & Francis Group
Collection
14
Is part of
Journal of Oral Microbiology
Citation
Moussa, Dina; Sharma, Ashok K.; Mansour, Tamer [et al.]. Functional signatures of ex-vivo dental caries onset. Journal of Oral Microbiology, 2022, 14(1), 2123624. Disponible en: <https://www.tandfonline.com/doi/full/10.1080/20002297.2022.2123624>. Fecha de acceso: 19 abr. 2023. DOI: 10.1080/20002297.2022.2123624
Link to the related item
This item appears in the following Collection(s)
- Odontologia [245]
Rights
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/