Functional signatures of ex-vivo dental caries onset
Autor/a
Sharma, Ashok K.
Witthuhn, Bruce
Rudney, Joel D.
Aparicio, Conrado
Data de publicació
2022ISSN
2000-2297
Resum
Background: The etiology of dental caries remains poorly understood. With the advent of next-generation sequencing, a number of studies have focused on the microbial ecology of the disease. However, taxonomic associations with caries have not been consistent. Researchers have also pursued function-centric studies of the caries microbial communities aiming to identify consistently conserved functional pathways. A major question is whether changes in microbiome are a cause or a consequence of the disease. Thus, there is a critical need to define conserved functional signatures at the onset of dental caries. Methods: Since it is unethical to induce carious lesions clinically, we developed an innovative longitudinal ex-vivo model integrated with the advanced non-invasive multiphoton second harmonic generation bioimaging to spot the very early signs of dental caries, combined with 16S rRNA short amplicon sequencing and liquid chromatography-mass spectrometry-based targeted metabolomics. Findings: For the first time, we induced longitudinally monitored caries lesions validated with the scanning electron microscope. Consequently, we spotted the caries onset and, associated with it, distinguished five differentiating metabolites – Lactate, Pyruvate, Dihydroxyacetone phosphate, Glyceraldehyde 3-phosphate (upregulated) and Fumarate (downregulated). Those metabolites co-occurred with certain bacterial taxa; Streptococcus, Veillonella, Actinomyces, Porphyromonas, Fusobacterium, and Granulicatella, regardless of the abundance of other taxa.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
616.3 - Patologia de l'aparell digestiu. Odontologia
Paraules clau
Càries dental
Model longitudinal
Signatures
Metabolòmica
Genòmica
Bioimatge no invasiva
Caries dental
Modelo longitudinal
Firmas
Metabolómica
Genómica
Bioimagen no invasiva
Dental caries
Longitudinal model
Signatures
Metabolomics
Genomics
Non-invasive bioimaging
Pàgines
20
Publicat per
Taylor & Francis Group
Col·lecció
14
Publicat a
Journal of Oral Microbiology
Citació
Moussa, Dina; Sharma, Ashok K.; Mansour, Tamer [et al.]. Functional signatures of ex-vivo dental caries onset. Journal of Oral Microbiology, 2022, 14(1), 2123624. Disponible en: <https://www.tandfonline.com/doi/full/10.1080/20002297.2022.2123624>. Fecha de acceso: 19 abr. 2023. DOI: 10.1080/20002297.2022.2123624
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Drets
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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