dc.contributor.author | Moussa, Dina | |
dc.contributor.author | Sharma, Ashok K. | |
dc.contributor.author | Mansour, Tamer | |
dc.contributor.author | Witthuhn, Bruce | |
dc.contributor.author | Perdigao, Jorge | |
dc.contributor.author | Rudney, Joel D. | |
dc.contributor.author | Aparicio, Conrado | |
dc.contributor.author | Gomez, Andres | |
dc.date.accessioned | 2023-04-19T14:10:40Z | |
dc.date.available | 2023-04-19T14:10:40Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Moussa, Dina; Sharma, Ashok K.; Mansour, Tamer [et al.]. Functional signatures of ex-vivo dental caries onset. Journal of Oral Microbiology, 2022, 14(1), 2123624. Disponible en: <https://www.tandfonline.com/doi/full/10.1080/20002297.2022.2123624>. Fecha de acceso: 19 abr. 2023. DOI: 10.1080/20002297.2022.2123624 | ca |
dc.identifier.issn | 2000-2297 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/3673 | |
dc.description.abstract | Background: The etiology of dental caries remains poorly understood. With the advent of next-generation sequencing, a number of studies have focused on the microbial ecology of the disease. However, taxonomic associations with caries have not been consistent. Researchers have also pursued function-centric studies of the caries microbial communities aiming to identify consistently conserved functional pathways. A major question is whether changes in microbiome are a cause or a consequence of the disease. Thus, there is a critical need to define conserved functional signatures at the onset of dental caries. Methods: Since it is unethical to induce carious lesions clinically, we developed an innovative longitudinal ex-vivo model integrated with the advanced non-invasive multiphoton second harmonic generation bioimaging to spot the very early signs of dental caries, combined with 16S rRNA short amplicon sequencing and liquid chromatography-mass spectrometry-based targeted metabolomics. Findings: For the first time, we induced longitudinally monitored caries lesions validated with the scanning electron microscope. Consequently, we spotted the caries onset and, associated with it, distinguished five differentiating metabolites – Lactate, Pyruvate, Dihydroxyacetone phosphate, Glyceraldehyde 3-phosphate (upregulated) and Fumarate (downregulated). Those metabolites co-occurred with certain bacterial taxa; Streptococcus, Veillonella, Actinomyces, Porphyromonas, Fusobacterium, and Granulicatella, regardless of the abundance of other taxa. | en |
dc.format.extent | 20 | ca |
dc.language.iso | eng | ca |
dc.publisher | Taylor & Francis Group | ca |
dc.relation.ispartof | Journal of Oral Microbiology | ca |
dc.relation.ispartofseries | 14 | |
dc.relation.uri | https://www.tandfonline.com/doi/full/10.1080/20002297.2022.2123624 | ca |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject.other | Càries dental | ca |
dc.subject.other | Model longitudinal | ca |
dc.subject.other | Signatures | ca |
dc.subject.other | Metabolòmica | ca |
dc.subject.other | Genòmica | ca |
dc.subject.other | Bioimatge no invasiva | ca |
dc.subject.other | Caries dental | es |
dc.subject.other | Modelo longitudinal | es |
dc.subject.other | Firmas | es |
dc.subject.other | Metabolómica | es |
dc.subject.other | Genómica | es |
dc.subject.other | Bioimagen no invasiva | es |
dc.subject.other | Dental caries | en |
dc.subject.other | Longitudinal model | en |
dc.subject.other | Signatures | en |
dc.subject.other | Metabolomics | en |
dc.subject.other | Genomics | en |
dc.subject.other | Non-invasive bioimaging | en |
dc.title | Functional signatures of ex-vivo dental caries onset | en |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 616.3 | ca |
dc.identifier.doi | https://dx.doi.org/10.1080/20002297.2022.2123624 | ca |