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dc.contributor.authorBosch Rué, Elia
dc.contributor.authorDíez-Tercero, Leire
dc.contributor.authorDelgado, Luis Maria
dc.contributor.authorPerez, Roman
dc.date.accessioned2022-07-04T14:42:04Z
dc.date.available2022-07-04T14:42:04Z
dc.date.issued2022
dc.identifier.citationBosch Rué, Elia; Díez-Tercero, Leire; Delgado, Luis Maria [et al.]. Biofabrication of collagen tissue-engineered blood vessels with direct co-axial extrusion. International Journal of Molecular Sciences, 2022, 23(10), 5618. Disponible en: <https://www.mdpi.com/1422-0067/23/10/5618>. Fecha de acceso: 4 jul. 2022. DOI: 10.3390/ijms23105618ca
dc.identifier.issn1422-0067ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/3348
dc.description.abstractCardiovascular diseases are considered one of the worldwide causes of death, with atherosclerosis being the most predominant. Nowadays, the gold standard treatment is blood vessel replacement by bypass surgery; however, autologous source is not always possible. Thereby, tissue-engineered blood vessels (TEBVs) are emerging as a potential alternative source. In terms of composition, collagen has been selected in many occasions to develop TEBVs as it is one of the main extracellular matrix components of arteries. However, it requires specific support or additional processing to maintain the tubular structure and appropriate mechanical properties. Here, we present a method to develop support-free collagen TEBVs with co-axial extrusion in a one-step procedure with high concentrated collagen. The highest concentration of collagen of 20 mg/mL presented a burst pressure of 619.55 ± 48.77 mmHg, being able to withstand perfusion of 10 dynes/cm2. Viability results showed a high percentage of viability (86.1 and 85.8% with 10 and 20 mg/mL, respectively) of human aortic smooth muscle cells (HASMCs) and human umbilical vein endothelial cells (HUVEC) after 24 h extrusion. Additionally, HUVEC and HASMCs were mainly localized in their respective layers, mimicking the native distribution. All in all, this approach allows the direct extrusion of collagen TEBVs in a one-step procedure with enough mechanical properties to be perfused.ca
dc.format.extent12ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofInternational Journal of Molecular Sciencesca
dc.relation.ispartofseries23;10
dc.relation.urihttps://www.mdpi.com/1422-0067/23/10/5618ca
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citedca
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.otherVasos sanguinis d'enginyeria de teixitsca
dc.subject.otherCol·lagenca
dc.subject.otherExtrusió coaxialca
dc.subject.otherConstrucció vascularca
dc.subject.otherHidrogels carregats de cèl·lulesca
dc.subject.otherVasos sanguíneos de ingeniería tisulares
dc.subject.otherColágenoes
dc.subject.otherExtrusión coaxiales
dc.subject.otherConstrucción vasculares
dc.subject.otherHidrogeles cargados de célulases
dc.subject.otherTissue-engineered blood vesselsen
dc.subject.otherCollagenen
dc.subject.otherCo-axial extrusionen
dc.subject.otherVascular constructen
dc.subject.otherCell-laden hydrogelsen
dc.titleBiofabrication of collagen tissue-engineered blood vessels with direct co-axial extrusionen
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc61ca
dc.identifier.doihttps://dx.doi.org/10.3390/ijms23105618ca


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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/