Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration
Author
Bosch Rué, Elia
Díez-Tercero, Leire
Rodríguez González, Raquel
Pérez Antoñanzas, Roman
Publication date
2021ISSN
1932-6203
Abstract
The use of copper (Cu2+) and cobalt (Co2+) has been described to stimulate blood vessel formation, a key process for the success of tissue regeneration. However, understanding how different concentrations of these ions affect cellular response is important to design scaffolds for their delivery to better fine tune the angiogenic response. On the one hand, gene expression analysis and the assessment of tubular formation structures with human umbilical vein endothelial cells (HUVEC) revealed that high concentrations (10μM) of Cu2+ in early times and lower concentrations (0.1 and 1μM) at later times (day 7) enhanced angiogenic response. On the other hand, higher concentrations (25μM) of Co2+ during all time course increased the angiogenic gene expression and 0.5, 5 and 25μM enhanced the ability to form tubular structures. To further explore synergistic effects combining both ions, the non-toxic concentrations were used simultaneously, although results showed an increased cell toxicity and no improvement of angiogenic response. These results provide useful information for the design of Cu2+ or Co2+ delivery scaffolds in order to release the appropriate concentration during time course for blood vessel stimulation.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Coure
Cobalt
Formació de vasos sanguinis
Regeneració dels teixits
Ions
Resposta cel·lular
Resposta angiogènica
Angiogènesi
Cobre
Cobalto
Formación de vasos sanguíneos
Regeneración de los tejidos
Iones
Respuesta celular
Respuesta angiogénica
Angiogénesis
Copper
Cobalt
Formation of blood vessels
Tissue regeneration
Ions
Cellular response
Angiogenic response
Angiogenesis
Pages
21
Publisher
Public Library of Science
Collection
16;10
Is part of
PLoS One
Citation
Bosch-Rue, Elia; Díez-Tercero, Leire; Rodríguez-González, Raquel [et al.]. Assessing the potential role of copper and cobalt in stimulating angiogenesis for tissue regeneration. PLoS One, 2021, 16(10), e0259125. Disponible en: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0259125>. Fecha de acceso: 2 nov. 2021. DOI: 10.1371/journal.pone.0259125
This item appears in the following Collection(s)
- Ciències Bàsiques [65]
Rights
© 2021 Bosch-Rué et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/