Browsing Ciències Bàsiques by Subject "Biología sintética"
Now showing items 1-4 of 4
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Engineered live bacteria suppress Pseudomonas aeruginosa infection in mouse lung and dissolve endotracheal-tube biofilms
(Nature Biotechnology, 2023)Engineered live bacteria could provide a new modality for treating lung infections, a major cause of mortality worldwide. In the present study, we engineered a genome-reduced human lung bacterium, ... -
Engineering a genome reduced bacterium to eliminate Staphylococcus aureus biofilms in vivo
(Molecular Systems Biology, 2021)Bacteria present a promising delivery system for treating human diseases. Here, we engineered the genome-reduced human lung pathogen Mycoplasma pneumoniae as a live biotherapeutic to treat biofilm-associated ... -
LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions
(Microbial Biotechnology, 2021)The removal of unwanted genetic material is a key aspect in many synthetic biology efforts and often requires preliminary knowledge of which genomic regions are dispensable. Typically, these efforts are ... -
SURE editing: combining oligo-recombineering and programmable insertion/deletion of selection markers to efficiently edit the Mycoplasma pneumoniae genome
(Nucleic Acids Research, 2022)The development of advanced genetic tools is boosting microbial engineering which can potentially tackle wide-ranging challenges currently faced by our society. Here we present SURE editing, a ...