Browsing Ciències Bàsiques by Author "Miravet-Verde, Samuel"
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LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions
Shaw, Daniel; Miravet-Verde, Samuel; Piñero Lambea, Carlos; Serrano, Luis; Lluch, Maria (Microbial Biotechnology, 2021)The removal of unwanted genetic material is a key aspect in many synthetic biology efforts and often requires preliminary knowledge of which genomic regions are dispensable. Typically, these efforts are ... -
SURE editing: combining oligo-recombineering and programmable insertion/deletion of selection markers to efficiently edit the Mycoplasma pneumoniae genome
Piñero Lambea, Carlos; Eva, Garcia-Ramallo; Miravet-Verde, Samuel; Burgos, Raul; Scarpa, Margherita; Serrano, Luis; Lluch-Senar, Maria (Nucleic Acids Research, 2022)The development of advanced genetic tools is boosting microbial engineering which can potentially tackle wide-ranging challenges currently faced by our society. Here we present SURE editing, a ...