Browsing Ciències Bàsiques by Author "Huguet Casades, Josep Maria"

Now showing items 1-10 of 10

    • Counter-propagating dual-trap optical tweezers based on linear momentum conservation 

      Ribezzi-Crivellari, M.; Huguet Casades, Josep Maria; Ritort, F. (Review of Scientific Instruments, 2013)
      We present a dual-trap optical tweezers setup which directly measures forces using linear momentum conservation. The setup uses a counter-propagating geometry, which allows momentum measurement on each ...

    • Derivation of nearest-neighbor DNA parameters in magnesium from single molecule experiments 

      Huguet Casades, Josep Maria; Ribezzi-Crivellari, Marco; Valim Bizarro, Cristiano; Ritort, Felix (Nucleic Acids Research, 2017)
      DNA hybridization is an essential molecular reaction in biology with many applications. The nearest-neighbor (NN) model for nucleic acids predicts DNA thermodynamics using energy values for the different ...

    • Dynamic force spectroscopy of DNA hairpins: I. Force kinetics and free energy landscapes 

      Mossa, A.; Manosas, M.; Forns, N.; Huguet Casades, Josep Maria; Ritort, F. (Journal of Statistical Mechanics: Theory and Experiment, 2009)
      We investigate the thermodynamics and kinetics of DNA hairpins that fold/unfold under the action of applied mechanical force. We introduce the concept of the molecular free energy landscape and derive ...

    • Dynamic force spectroscopy of DNA hairpins: II. Irreversibility and dissipation 

      Manosas, M.; Mossa, A.; Forns, N.; Huguet Casades, Josep Maria; Ritort, F. (Journal of Statistical Mechanics: Theory and Experiment, 2009)
      We investigate irreversibility and dissipation in single molecules that cooperatively fold/unfold in a two-state manner under the action of mechanical force. We apply path thermodynamics to derive ...

    • Improving signal/noise resolution in single-molecule experiments using molecular constructs with short handles 

      Forns, N.; de Lorenzo, S.; Manosas, M.; Hayashi, K.; Huguet Casades, Josep Maria; Ritort, F. (Biophysical Journal, 2011)
      We investigate unfolding/folding force kinetics in DNA hairpins exhibiting two and three states with newly designed short dsDNA handles (29 bp) using optical tweezers. We show how the higher stiffness ...

    • Investigating the thermodynamics of small biosystems with optical tweezers 

      Mossa, Alessandro; Huguet Casades, Josep Maria; Ritort, Felix (Physica E: Low-dimensional Systems and Nanostructures, 2010)
      We present two examples of how single-molecule experimental techniques applied to biological systems can give insight into problems within the scope of equilibrium and nonequilibrium mesoscopic ...

    • Measurement of work in single-molecule pulling experiments 

      Mossa, Alessandro; de Lorenzo, Sara; Huguet Casades, Josep Maria; Ritort, Felix (The Journal of Chemical Physics, 2009)
      A main goal of single-molecule experiments is to evaluate equilibrium free energy differences by applying fluctuation relations to repeated work measurements along irreversible processes. We quantify ...

    • Single-molecule derivation of salt dependent base-pair free energies in DNA 

      Huguet Casades, Josep Maria (Proceedings of the National Academy of Sciences., 2010)
      Accurate knowledge of the thermodynamic properties of nucleic acids is crucial to predicting their structure and stability. To date most measurements of base-pair free energies in DNA are obtained in ...

    • Single-molecule stochastic resonance 

      Hayashi, K.; de Lorenzo, S.; Manosas, M.; Huguet Casades, Josep Maria; Ritort, F. (Physical Review X, 2012)
      Stochastic resonance (SR) is a well-known phenomenon in dynamical systems. It consists of the amplification and optimization of the response of a system assisted by stochastic (random or probabilistic) ...

    • Work distributions in the T = 0 random field Ising model 

      Illa, Xavier; Huguet Casades, Josep Maria; Vives, Eduard (Physical Review E, 2008)
      We perform a numerical study of the three-dimensional random-field Ising model at T= 0. We compare work distributions along metastable trajectories obtained with the single-spin flip dynamics with the ...