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dc.contributor.authorMas-Moruno, Carlos
dc.contributor.authorGarrido, Beatriz
dc.contributor.authorRodriguez, Daniel
dc.contributor.authorRuperez, Elisa
dc.contributor.authorGil Mur, Francisco Javier
dc.date.accessioned2021-05-14T14:57:10Z
dc.date.available2021-05-14T14:57:10Z
dc.date.issued2015
dc.identifier.citationMas-Moruno, Carlos; Garrido, Beatriz; Rodriguez, Daniel [et al.]. Biofunctionalization strategies on tantalum-based materials for osseointegrative applications. Journal of Materials Science: Materials in Medicine, 2015, 26, 109. Disponible en: <https://link.springer.com/article/10.1007/s10856-015-5445-z>. Fecha de acceso: 14 may. 2021. DOI: 10.1007/s10856-015-5445-zca
dc.identifier.issn1573-4838ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/2541
dc.description.abstractThe use of tantalum as biomaterial for orthopedic applications is gaining considerable attention in the clinical practice because it presents an excellent chemical stability, body fluid resistance, biocompatibility, and it is more osteoconductive than titanium or cobalt-chromium alloys. Nonetheless, metallic biomaterials are commonly bioinert and may not provide fast and long-lasting interactions with surrounding tissues. The use of short cell adhesive peptides derived from the extracellular matrix has shown to improve cell adhesion and accelerate the implant’s biointegration in vivo. However, this strategy has been rarely applied to tantalum materials. In this work, we have studied two immobilization strategies (physical adsorption and covalent binding via silanization) to functionalize tantalum surfaces with a cell adhesive RGD peptide. Surfaces were used untreated or activated with either HNO3 or UV/ozone treatments. The process of biofunctionalization was characterized by means of physicochemical and biological methods. Physisorption of the RGD peptide on control and HNO3-treated tantalum surfaces significantly enhanced the attachment and spreading of osteoblast-like cells; however, no effect on cell adhesion was observed in ozone-treated samples. This effect was attributed to the inefficient binding of the peptide on these highly hydrophilic surfaces, as evidenced by contact angle measurements and X-ray photoelectron spectroscopy. In contrast, activation of tantalum with UV/ozone proved to be the most efficient method to support silanization and subsequent peptide attachment, displaying the highest values of cell adhesion. This study demonstrates that both physical adsorption and silanization are feasible methods to immobilize peptides onto tantalum-based materials, providing them with superior bioactivity.en
dc.format.extent12ca
dc.language.isoengca
dc.publisherSpringer Natureca
dc.relation.ispartofJournal of Materials Science: Materials in Medicineca
dc.relation.ispartofseries26;
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.ca
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.otherMaterials biomèdicsca
dc.subject.otherOrtopèdiaca
dc.subject.otherTitanica
dc.subject.otherCobaltca
dc.subject.otherCromca
dc.subject.otherImplants artificialsca
dc.subject.otherBiocompatibilitatca
dc.subject.otherMateriales biomédicoses
dc.subject.otherOrtopediaes
dc.subject.otherTitanioes
dc.subject.otherCobaltoes
dc.subject.otherCromoes
dc.subject.otherImplantes artificialeses
dc.subject.otherBiocompatibilidades
dc.subject.otherBiomedical materialsen
dc.subject.otherOrthopedicsen
dc.subject.otherTitaniumen
dc.subject.otherCobalten
dc.subject.otherChromeen
dc.subject.otherArtificial implantsen
dc.subject.otherBiocompatibilityen
dc.titleBiofunctionalization strategies on tantalum-based materials for osseointegrative applicationsen
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/6PN/MAT2012-30706ca
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/321985ca
dc.subject.udc61ca
dc.subject.udc620ca
dc.identifier.doihttps://dx.doi.org/10.1007/s10856-015-5445-zca


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This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/