Direct extrusion of individually encapsulated endothelial and smooth muscle cells mimicking blood vessel structures and vascular native cell alignment
Author
Bosch-Rué, Elia
Delgado, Luis M.
Gil Mur, Francisco Javier
Pérez Antoñanzas, Roman
Publication date
2020ISSN
1758-5082
Abstract
Cardiovascular diseases (CVDs) are considered the principal cause of worldwide death, being atherosclerosis the main etiology. Up to now, the predominant treatment for CVDs has been bypass surgery from autologous source. However, due to previous harvest or the type of disease, this is not always an option. For this reason, tissue engineered blood vessels (TEBV) emerged as an alternative graft source for blood vessel replacement. In order to develop a TEBV, it should mimic the architecture of a native blood vessel encapsulating the specific vascular cells in their respective layers with native alignment, and with appropriate mechanical stability. Here, we propose the extrusion of two different cell encapsulating hydrogels, mainly alginate and collagen, and a sacrificial polymer, through a triple coaxial nozzle, which in contact with a crosslinking solution allows the formation of bilayered hollow fibers, mimicking the architecture of native blood vessels. Prior to extrusion, the innermost cell encapsulating hydrogel was loaded with human umbilical vein endothelial cells (HUVECs), whereas the outer hydrogel was loaded with human aortic smooth muscle cells (HASMCs). The size of the TEVB could be controlled by changing the injection speed, presenting homogeneity between the constructs. The obtained structures were robust, allowing its manipulation as well as the perfusion of liquids. Both cell types presented high rates of survival after the extrusion process as well as after 20 d in culture (over 90%). Additionally, a high percentage of HASMC and HUVEC were aligned perpendicular and parallel to the TEBV, respectively, in their own layers, resembling the physiological arrangement found in vivo. Our approach enables the rapid formation of TEBV-like structures presenting high cell viability and allowing proliferation and natural alignment of vascular cells.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Vasos sanguinis
Sistema cardiovascular -- Malalties
Aterosclerosi
Cirurgia
Vasos sanguíneos
Aparato circulatorio -- Enfermedades
Aterosclerosis
Cirugía
Blood -- vessels
Cardiovascular system -- Diseases
Atherosclerosis
Surgery
Pages
15
Publisher
IOP Science
Collection
13;
Is part of
Biofabrication
Citation
Bosch-Rué, E.; Delgado, Luis M.; Gil Mur, Francisco Javier [et al.]. Direct extrusion of individually encapsulated endothelial and smooth muscle cells mimicking blood vessel structures and vascular native cell alignment. Biofabrication, 2020, 13, 015003. Disponible en: <https://iopscience.iop.org/article/10.1088/1758-5090/abbd27#references>. Fecha de acceso: 25 feb. 2021. DOI: 10.1088/1758-5090/abbd27
Grant agreement number
info:eu-repo/grantAgreement/ES/2PE/RTI2018-096088-J-100
This item appears in the following Collection(s)
- Ciències de la Salut [739]
Rights
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Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/