Browsing Ciències de la Salut by Author "Galy, Bruno"
Now showing items 1-5 of 5
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Control of systemic iron homeostasis by the 3’ iron-responsive element of divalent metal transporter 1 in mice
Tybl, Elisabeth; Gunshin, Hiromi; Gupta, Sanjay; Barrientos, Tomasa; Bonadonna, Michael; Celma Nos, Ferran; Palais, Gael; Karim, Zoubida; Sanchez, Mayka; Andrews, Nancy C.; Galy, Bruno (HemaSphere, 2020)Tight control of intestinal iron absorption is required to avoid both iron insufficiency and excess.1 Dietary nonheme iron is taken up by absorptive enterocytes via the apical iron transporter DMT1 ... -
Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays
Sanchez, Mayka; Galy, Bruno; Hentze, Matthias W.; Muckenthaler, Martina U. (Nature Protocols, 2007)RNA-binding proteins (RBPs) frequently regulate the post-transcriptional fate of target mRNAs. To identify novel target mRNAs of RBPs, we incubate total RNA with recombinant RBP and immunoselect the ... -
Iron regulatory protein-1 and -2: transcriptome-wide definition of binding mRNAs and shaping of the cellular proteome by iron regulatory proteins
Sanchez, Mayka; Galy, Bruno; Schwanhaeusser, Bjoern; Blake, Jonathon; Bähr-Ivacevic, Tomi; Benes, Vladimir; Selbach, Matthias; Muckenthaler, Martina U.; Hentze, Matthias W. (Blood, 2011)Iron regulatory proteins (IRPs) 1 and 2 are RNA-binding proteins that control cellular iron metabolism by binding to conserved RNA motifs called iron-responsive elements (IREs). The currently known ... -
Iron-regulatory proteins limit hypoxia-inducible factor 2α expression in iron deficiency
Sanchez, Mayka; Galy, Bruno; Muckenthaler, Martina U.; Hentze, Matthias W. (Nature Structural & Molecular Biology, 2007)Hypoxia stimulates erythropoiesis, the major iron-utilization pathway. We report the discovery of a conserved, functional iron-responsive element (IRE) in the 5′ untranslated region of the messenger RNA ... -
The actin binding protein profilin 2 is a novel regulator of iron homeostasis
Luscieti, Sara; Galy, Bruno; Gutierrez, Lucia; Reinke, Michael; Couso, Jorge; Shvartsman, Maya; Di Pascale, Antonio; Witke, Walter; Hentze, Matthias W.; Pilo Boyl, Pietro; Sanchez, Mayka (Blood, 2017)Cellular iron homeostasis is controlled by the iron regulatory proteins (IRPs) 1 and 2 that bind cis-regulatory iron-responsive elements (IRE) on target messenger RNAs (mRNA). We identified profilin 2 ...