Profiling of RNA structure at single-nucleotide resolution using nextPARS
Fecha de publicación
2021ISBN
978-1-0716-1306-1
Resumen
RNA molecules play important roles in almost every cellular process, and their functions are mediated by their sequence and structure. Determining the secondary structure of RNAs is central to understanding RNA function and evolution. RNA structure probing techniques coupled to high-throughput sequencing allow determining structural features of RNA molecules at transcriptome-wide scales. Our group recently developed a novel Illumina-based implementation of in vitro parallel probing of RNA structures called nextPARS. Here, we describe a protocol for the computation of the nextPARS scores and their use to obtain the structural profile (single- or double-stranded state) of an RNA sequence at single-nucleotide resolution.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
57 - Biología
Palabras clave
Páginas
11
Publicado por
Springer Nature
Publicado en
RNA Bioinformatics
Citación
Chorostecki, Uciel Pablo; Willis, Jesse R.; Saus, Ester [et al.]. Profiling of RNA structure at single-nucleotide resolution using nextPARS. En: RNA Bioinformatics, Springer Nature, 2021. ISBN: 978-1-0716-1306-1. DOI: 10.1007/978-1-0716-1307-8_4
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This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
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