Mostra el registre parcial de l'element
Counter-propagating dual-trap optical tweezers based on linear momentum conservation
dc.contributor.author | Ribezzi-Crivellari, M. | |
dc.contributor.author | Huguet Casades, Josep Maria | |
dc.contributor.author | Ritort, F. | |
dc.date.accessioned | 2024-03-18T09:04:08Z | |
dc.date.available | 2024-03-18T09:04:08Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Ribezzi-Crivellari, M.; Huguet Casades, Josep Maria; Ritort, F. Counter-propagating dual-trap optical tweezers based on linear momentum conservation. Review of Scientific Instruments, 2013, 84, 043104. Disponible en: <https://pubs.aip.org/aip/rsi/article-abstract/84/4/043104/358115/Counter-propagating-dual-trap-optical-tweezers?redirectedFrom=fulltext>. Fecha de acceso: 18 mar. 2024. DOI: 10.1063/1.4799289 | ca |
dc.identifier.issn | 0034-6748 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/4159 | |
dc.description.abstract | We present a dual-trap optical tweezers setup which directly measures forces using linear momentum conservation. The setup uses a counter-propagating geometry, which allows momentum measurement on each beam separately. The experimental advantages of this setup include low drift due to all-optical manipulation, and a robust calibration (independent of the features of the trapped object or buffer medium) due to the force measurement method. Although this design does not attain the high-resolution of some co-propagating setups, we show that it can be used to perform different single molecule measurements: fluctuation-based molecular stiffness characterization at different forces and hopping experiments on molecular hairpins. Remarkably, in our setup it is possible to manipulate very short tethers (such as molecular hairpins with short handles) down to the limit where beads are almost in contact. The setup is used to illustrate a novel method for measuring the stiffness of optical traps and tethers on the basis of equilibrium force fluctuations, i.e., without the need of measuring the force vs molecular extension curve. This method is of general interest for dual trap optical tweezers setups and can be extended to setups which do not directly measure forces. | ca |
dc.format.extent | Desconocido | ca |
dc.language.iso | eng | ca |
dc.publisher | AIP Publishing | ca |
dc.relation.ispartof | Review of Scientific Instruments | ca |
dc.relation.ispartofseries | 84 | |
dc.rights | © Copyright 2024 AIP Publishing LLC | ca |
dc.subject.other | Mecánica newtoniana | ca |
dc.subject.other | Ruido electrónico | ca |
dc.subject.other | Fibra óptica | ca |
dc.subject.other | Óptica geométrica | ca |
dc.subject.other | Propiedades ópticas | ca |
dc.subject.other | Pinzas ópticas | ca |
dc.subject.other | Autoenfoque | ca |
dc.subject.other | Trampa óptica | ca |
dc.subject.other | Física láser | ca |
dc.subject.other | Técnicas de molécula única | ca |
dc.subject.other | Mecànica newtoniana | ca |
dc.subject.other | Soroll electrònic | ca |
dc.subject.other | Fibra òptica | ca |
dc.subject.other | Òptica geomètrica | ca |
dc.subject.other | Propietats òptiques | ca |
dc.subject.other | Pinces òptiques | ca |
dc.subject.other | Autoenfocament | ca |
dc.subject.other | Trampa òptica | ca |
dc.subject.other | Física làser | ca |
dc.subject.other | Tècniques de molècula única | ca |
dc.subject.other | Newtonian mechanics | ca |
dc.subject.other | Electronic noise | ca |
dc.subject.other | Fiber optics | ca |
dc.subject.other | Geometrical optics | ca |
dc.subject.other | Optical properties | ca |
dc.subject.other | Optical tweezers | ca |
dc.subject.other | Self focusing | ca |
dc.subject.other | Optical trapping | ca |
dc.subject.other | Laser physics | ca |
dc.subject.other | Single molecule techniques | ca |
dc.title | Counter-propagating dual-trap optical tweezers based on linear momentum conservation | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 611 | ca |
dc.identifier.doi | https://dx.doi.org/10.1063/1.4799289 | ca |
Fitxers en aquest element
Fitxers | Grandària | Format | Visualització |
---|---|---|---|
No hi ha fitxers associats a aquest element. |
Aquest element apareix en la col·lecció o col·leccions següent(s)
-
Ciències Bàsiques [65]