Efficiency and precision of microRNA biogenesis modes in plants
Autor/a
Moro, Belén
Chorostecki, Uciel
Arikit, Siwaret
Suarez, Irina P.
Höbartner, Claudia
Rasia, Rodolfo M.
Meyers, Blake C.
Palatnik, Javier F.
Fecha de publicación
2018ISSN
0305-1048
Resumen
Many evolutionarily conserved microRNAs (miRNAs) in plants regulate transcription factors with key functions in development. Hence, mutations in the core components of the miRNA biogenesis machinery cause strong growth defects. An essential aspect of miRNA biogenesis is the precise excision of the small RNA from its precursor. In plants, miRNA precursors are largely variable in size and shape and can be processed by different modes. Here, we optimized an approach to detect processing intermediates during miRNA biogenesis. We characterized a miRNA whose processing is triggered by a terminal branched loop. Plant miRNA processing can be initiated by internal bubbles, small terminal loops or branched loops followed by dsRNA segments of 15–17 bp. Interestingly, precision and efficiency vary with the processing modes. Despite the various potential structural determinants present in a single a miRNA precursor, DCL1 is mostly guided by a predominant structural region in each precursor in wild-type plants. However, our studies in fiery1, hyl1 and se mutants revealed the existence of cleavage signatures consistent with the recognition of alternative processing determinants. The results provide a general view of the mechanisms underlying the specificity of miRNA biogenesis in plants.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
5 - Ciencias puras y naturales
Palabras clave
Ciència i matemàtiques
Ciencias y Matemáticas
Science and mathematics
Páginas
14
Publicado por
Oxford University Press
Colección
46; 20
Publicado en
Nucleic Acids Research
Citación
Moro, Belén; Chorostecki, Uciel; Arikit, Siwaret [et al.]. Efficiency and precision of microRNA biogenesis modes in plants. Nucleic Acids Research, 2018, 46(10), p. 10709-10723. Disponible en: <https://academic.oup.com/nar/article/46/20/10709/5115824>. Fecha de acceso: 7 feb. 2024. DOI: 10.1093/nar/gky853
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Derechos
© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.