dc.contributor.author | Rojas, Arantxa M. L. | |
dc.contributor.author | Drusin, Salvador I. | |
dc.contributor.author | Chorostecki, Uciel | |
dc.contributor.author | Mateos, Julieta L. | |
dc.contributor.author | Moro, Belén | |
dc.contributor.author | Bologna, Nicolas G. | |
dc.contributor.author | Bresso, Edgardo G. | |
dc.contributor.author | Schapire, Arnaldo | |
dc.contributor.author | Rasia, Rodolfo M. | |
dc.contributor.author | Moreno, Diego M. | |
dc.contributor.author | Palatnik, Javier F. | |
dc.date.accessioned | 2024-02-07T15:27:14Z | |
dc.date.available | 2024-02-07T15:27:14Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Rojas, Arantxa M. L.; Drusin, Salvador I.; Chorostecki, Uciel [et al.]. Identification of key sequence features required for microRNA biogenesis in plants. Nature Communications, 11, 5320. Disponible en: <https://www.nature.com/articles/s41467-020-19129-6>. Fecha de acceso: 7 feb. 2024. DOI: 10.1038/s41467-020-19129-6 | ca |
dc.identifier.issn | 2041-1723 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/4050 | |
dc.description.abstract | MicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop structure. In plants, the ribonuclease type III DICER-LIKE1 assisted by accessory proteins cleaves the precursor to release the mature miRNA. Numerous studies highlight the role of the precursor secondary structure during plant miRNA biogenesis; however, little is known about the relevance of the precursor sequence. Here, we analyzed the sequence composition of plant miRNA primary transcripts and found specifically located sequence biases. We show that changes in the identity of specific nucleotides can increase or abolish miRNA biogenesis. Most conspicuously, our analysis revealed that the identity of the nucleotides at unpaired positions of the precursor plays a crucial role during miRNA biogenesis in Arabidopsis. | ca |
dc.format.extent | 11 | ca |
dc.language.iso | eng | ca |
dc.publisher | Springer Nature | ca |
dc.relation.ispartof | Nature Communications | ca |
dc.relation.ispartofseries | 11 | |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | ca |
dc.subject.other | ARNs no codificants | ca |
dc.subject.other | Biologia molecular vegetal | ca |
dc.subject.other | Ciències vegetals | ca |
dc.subject.other | RNAi | ca |
dc.subject.other | ARN no codificantes | ca |
dc.subject.other | Biología molecular vegetal | ca |
dc.subject.other | Ciencias vegetales | ca |
dc.subject.other | ARNi | ca |
dc.subject.other | Non-coding RNAs | ca |
dc.subject.other | Plant molecular biology | ca |
dc.subject.other | Plant sciences | ca |
dc.subject.other | RNAi | ca |
dc.title | Identification of key sequence features required for microRNA biogenesis in plants | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 5 | ca |
dc.identifier.doi | https://dx.doi.org/10.1038/s41467-020-19129-6 | ca |