Novel strategies enhancing endodontic disinfection: antibacterial biodegradable calcium hydroxide nanoparticles in an ex vivo model
Author
Elmsmari, Firas
Delgado, Luis María
Duran-Sindreu, Fernando
A. Pérez, Román
García, Maria Luisa
Teulé Trull, Míriam
Afrashtehfar, Kelvin I.
González, José Antonio
Sánchez-López, Elena
Publication date
2023ISSN
0378-5173
Abstract
Due to the high failure rates associated to endodontic disinfection, this study aimed to investigate the antibacterial properties of poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with Ca(OH)2 for endodontic disinfection procedures. Ca(OH)2 NPs production and physicochemical characterization were carried out as well as multiple antibacterial tests using three bacterial strains and an ex vivo model of endodontic infection with extracted human teeth. Agar diffusion test and broth dilution determined the inhibition growth zones (n = 5) and the minimal inhibitory concentration (MIC, n = 5), respectively. Cell viability was assessed using Live/Dead staining with confocal microscopy (n = 5). Data was analysed using ANOVA followed by post-hoc analysis. After 24 h of incubation, Ca(OH)₂ NPs demonstrated a MIC of 10 µg/mL for Porphyromonas gingivalis (p < 0.001) and Enterococcus faecalis and 5 µg/mL for Fusobacterium nucleatum (p < 0.001). Although the agar diffusion test did not exhibit any inhibition area for Ca(OH)2 nor for Ca(OH)₂ NPs, this was probably due to the buffering effect of the agar medium. However, the antibacterial capacity was confirmed in an ex vivo model, where instrumentalized teeth were infected with Enterococcus Faecalis and treated after 28 days of culture. A significant reduction in bacterial metabolic activity was confirmed for Ca(OH)2 NPs (40 % reduction with a single dose) and confirmed by Live/Dead staining. In conclusion, Ca(OH)₂-loaded PLGA NPs present promising antibacterial efficacy for endodontic disinfection procedures.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Agents antibacterians
Hidròxid de calci
Desinfecció
Endodòncia
Enterococcus faecalis
Nanopartícules
Model antibacterià in vitro
Agentes antibacterianos
Hidróxido de calcio
Desinfección
Endodoncia
Enterococcus faecalis
Nanopartículas
Modelo antibacteriano in vitro
Anti-bacterial agents
Calcium hydroxide
Disinfection
Endodontics
Enterococcus faecalis
Nanoparticles in vitro antibacterial model
Pages
9
Publisher
Elsevier
Collection
648
Is part of
International Journal of Pharmaceutics
Citation
Elmsmari, Firas; Delgado, Luis María; Duran-Sindreu, Fernando [et al.]. Novel strategies enhancing endodontic disinfection: antibacterial biodegradable calcium hydroxide nanoparticles in an ex vivo model. International Journal of Pharmaceutics, 2023, 648, 123627. Disponible en: <https://www.sciencedirect.com/science/article/pii/S0378517323010499?via%3Dihub>. Fecha de acceso: 20 dic. 2023. DOI: 10.1016/j.ijpharm.2023.123627
This item appears in the following Collection(s)
- Odontologia [248]
Rights
Under a Creative Commons license.
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/