Implant surface physicochemistry affects keratinocyte hemidesmosome formation
Author
Raptopoulos, Michail
Fischer, Nicholas G.
Aparicio, Conrado
Publication date
2023ISSN
1552-4965
Abstract
Previous studies have shown hydrophilic/hydrophobic implant surfaces stimulate/hinder osseointegration. An analogous concept was applied here using common biological functional groups on a model surface to promote oral keratinocytes (OKs) proliferation and hemidesmosomes (HD) to extend implant lifespans through increased soft tissue attachment. However, it is unclear what physicochemistry stimulates HDs. Thus, common biological functional groups (NH2, OH, and CH3) were functionalized on glass using silanization. Non-functionalized plasma-cleaned glass and H silanization were controls. Surface modifications were confirmed with X-ray photoelectron spectroscopy and water contact angle. The amount of bovine serum albumin (BSA) and fibrinogen, and BSA thickness, were assessed to understand how adsorbed protein properties were influenced by physicochemistry and may influence HDs. OKs proliferation was measured, and HDs were quantified with immunofluorescence for collagen XVII and integrin β4. Plasma-cleaned surfaces were the most hydrophilic group overall, while CH3 was the most hydrophobic and OH was the most hydrophilic among functionalized groups. Modification with the OH chemical group showed the highest OKs proliferation and HD expression. The OKs response on OH surfaces appeared to not correlate to the amount or thickness of adsorbed model proteins. These results reveal relevant surface physicochemical features to favor HDs and improve implant soft tissue attachment.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Adjunt
Hemidesmosoma
Implant
Queratinòcit
Organosilà
Fisicoquímica
Apego
Fisicoquímica
Hemidesmosoma
Implantes
Queratinocito
Organosilano
Attachment
Hemidesmosome
Implant
Keratinocyte
Organosilane
Physicochemistry
Pages
10
Publisher
John Wiley & Sons
Is part of
Journal of Biomedical Materials Research Part A
Citation
Raptopoulos, Michail; Fischer, Nicholas G.; Aparicio, Conrado. Implant surface physicochemistry affects keratinocyte hemidesmosome formation. Journal of Biomedical Materials Research Part A, 2023, p. 1-10. Disponible en: <https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.37486>. Fecha de acceso: 20 abr. 2023. DOI: 10.1002/jbm.a.37486.
Link to the related item
This item appears in the following Collection(s)
- Odontologia [228]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/