Implant surface physicochemistry affects keratinocyte hemidesmosome formation
Autor/a
Raptopoulos, Michail
Fischer, Nicholas G.
Aparicio, Conrado
Data de publicació
2023ISSN
1552-4965
Resum
Previous studies have shown hydrophilic/hydrophobic implant surfaces stimulate/hinder osseointegration. An analogous concept was applied here using common biological functional groups on a model surface to promote oral keratinocytes (OKs) proliferation and hemidesmosomes (HD) to extend implant lifespans through increased soft tissue attachment. However, it is unclear what physicochemistry stimulates HDs. Thus, common biological functional groups (NH2, OH, and CH3) were functionalized on glass using silanization. Non-functionalized plasma-cleaned glass and H silanization were controls. Surface modifications were confirmed with X-ray photoelectron spectroscopy and water contact angle. The amount of bovine serum albumin (BSA) and fibrinogen, and BSA thickness, were assessed to understand how adsorbed protein properties were influenced by physicochemistry and may influence HDs. OKs proliferation was measured, and HDs were quantified with immunofluorescence for collagen XVII and integrin β4. Plasma-cleaned surfaces were the most hydrophilic group overall, while CH3 was the most hydrophobic and OH was the most hydrophilic among functionalized groups. Modification with the OH chemical group showed the highest OKs proliferation and HD expression. The OKs response on OH surfaces appeared to not correlate to the amount or thickness of adsorbed model proteins. These results reveal relevant surface physicochemical features to favor HDs and improve implant soft tissue attachment.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
616.3 - Patologia de l'aparell digestiu. Odontologia
Paraules clau
Adjunt
Hemidesmosoma
Implant
Queratinòcit
Organosilà
Fisicoquímica
Apego
Fisicoquímica
Hemidesmosoma
Implantes
Queratinocito
Organosilano
Attachment
Hemidesmosome
Implant
Keratinocyte
Organosilane
Physicochemistry
Pàgines
10
Publicat per
John Wiley & Sons
Publicat a
Journal of Biomedical Materials Research Part A
Citació
Raptopoulos, Michail; Fischer, Nicholas G.; Aparicio, Conrado. Implant surface physicochemistry affects keratinocyte hemidesmosome formation. Journal of Biomedical Materials Research Part A, 2023, p. 1-10. Disponible en: <https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.37486>. Fecha de acceso: 20 abr. 2023. DOI: 10.1002/jbm.a.37486.
Enllaç al document relacionat
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Drets
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