Biomimetic mineralized hybrid scaffolds with antimicrobial peptides
Author
Ye, Zhou
Zhu, Xiao
Mutreja, Isha
Kumar Boda, Sunil
Fischer, Nicholas G.
Zhang, Anqi
Lui, Christine
Qi, Yipin
Publication date
2021-08ISSN
2452-199X
Abstract
Infection in hard tissue regeneration is a clinically-relevant challenge. Development of scaffolds with dual function for promoting bone/dental tissue growth and preventing bacterial infections is a critical need in the field. Here we fabricated hybrid scaffolds by intrafibrillar-mineralization of collagen using a biomimetic process and subsequently coating the scaffold with an antimicrobial designer peptide with cationic and amphipathic properties. The highly hydrophilic mineralized collagen scaffolds provided an ideal substrate to form a dense and stable coating of the antimicrobial peptides. The amount of hydroxyapatite in the mineralized fibers modulated the rheological behavior of the scaffolds with no influence on the amount of recruited peptides and the resulting increase in hydrophobicity. The developed scaffolds were potent by contact killing of Gram-negative Escherichia coli and Gram-positive Streptococcus gordonii as well as cytocompatible to human bone marrow-derived mesenchymal stromal cells. The process of scaffold fabrication is versatile and can be used to control mineral load and/or intrafibrillar-mineralized scaffolds made of other biopolymers.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Mineralització biomimètica
Antimicrobiana
Pèptids catiònics i amfipàtics
Citocompatibilitat del teixit dur
Mineralización biomimética
Antimicrobiano
Péptidos catiónicos y anfipáticos
Citocompatibilidad de tejido duro
Biomimetic mineralization
Antimicrobial
Cationic and amphipathic peptides
Hard tissue cytocompatibility
Pages
11
Publisher
Elsevier
Collection
6; 8
Is part of
Bioactive Materials
Citation
Ye, Zhou; Zhu, Xiao; Mutreja, Isha [et al.]. Biomimetic mineralized hybrid scaffolds with antimicrobial peptides. Bioactive Materials, 2021, 6(8), p. 2250-2260. Disponible en: <https://www.sciencedirect.com/science/article/pii/S2452199X21000037>. Fecha de acceso: 20 abr. 2023. DOI: 10.1016/j.bioactmat.2020.12.029
Link to the related item
This item appears in the following Collection(s)
- Odontologia [245]
Rights
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/