Polydopamine incorporation enhances cell differentiation and antibacterial properties of 3D-printed guanosine-borate hydrogels for functional tissue regeneration
Publication date
2023ISSN
1422-0067
Abstract
Tissue engineering focuses on the development of materials as biosubstitutes that can be used to regenerate, repair, or replace damaged tissues. Alongside this, 3D printing has emerged as a promising technique for producing implants tailored to specific defects, which in turn increased the demand for new inks and bioinks. Especially supramolecular hydrogels based on nucleosides such as guanosine have gained increasing attention due to their biocompatibility, good mechanical characteristics, tunable and reversible properties, and intrinsic self-healing capabilities. However, most existing formulations exhibit insufficient stability, biological activity, or printability. To address these limitations, we incorporated polydopamine (PDA) into guanosine-borate (GB) hydrogels and developed a PGB hydrogel with maximal PDA incorporation and good thixotropic and printability qualities. The resulting PGB hydrogels exhibited a well-defined nanofibrillar network, and we found that PDA incorporation increased the hydrogel’s osteogenic activity while having no negative effect on mammalian cell survival or migration. In contrast, antimicrobial activity was observed against the Gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis. Thus, our findings suggest that our PGB hydrogel represents a significantly improved candidate as a 3D-printed scaffold capable of sustaining living cells, which may be further functionalized by incorporating other bioactive molecules for enhanced tissue integration.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Hidrogels basats en nucleòsids
Guanosina i derivats
Impressió 3D
Polidopamina
Activitat antibacteriana
Hidrogeles a base de nucleósidos
Guanosina y derivados
Impresión 3D
Polidopamina
Actividad antibacterial
Nucleoside-based hydrogels
Guanosine and derivatives
3D printing
Polydopamine
Antibacterial activity
Pages
25
Publisher
MDPI
Collection
24; 4
Is part of
International Journal of Molecular Sciences
Citation
Merino Gómez, Maria; Gil, FJ; Perez, Roman [et al.]. Polydopamine incorporation enhances cell differentiation and antibacterial properties of 3D-printed guanosine-borate hydrogels for functional tissue regeneration. International Journal of Molecular Sciences, 2023, 24(4), 4224. Disponible en: <https://www.mdpi.com/1422-0067/24/4/4224>. Fecha de acceso: 27 mar. 2023. DOI: 10.3390/ijms24044224
Link to the related item
This item appears in the following Collection(s)
- Odontologia [248]
Rights
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/