Polydopamine incorporation enhances cell differentiation and antibacterial properties of 3D-printed guanosine-borate hydrogels for functional tissue regeneration
Fecha de publicación
2023ISSN
1422-0067
Resumen
Tissue engineering focuses on the development of materials as biosubstitutes that can be used to regenerate, repair, or replace damaged tissues. Alongside this, 3D printing has emerged as a promising technique for producing implants tailored to specific defects, which in turn increased the demand for new inks and bioinks. Especially supramolecular hydrogels based on nucleosides such as guanosine have gained increasing attention due to their biocompatibility, good mechanical characteristics, tunable and reversible properties, and intrinsic self-healing capabilities. However, most existing formulations exhibit insufficient stability, biological activity, or printability. To address these limitations, we incorporated polydopamine (PDA) into guanosine-borate (GB) hydrogels and developed a PGB hydrogel with maximal PDA incorporation and good thixotropic and printability qualities. The resulting PGB hydrogels exhibited a well-defined nanofibrillar network, and we found that PDA incorporation increased the hydrogel’s osteogenic activity while having no negative effect on mammalian cell survival or migration. In contrast, antimicrobial activity was observed against the Gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis. Thus, our findings suggest that our PGB hydrogel represents a significantly improved candidate as a 3D-printed scaffold capable of sustaining living cells, which may be further functionalized by incorporating other bioactive molecules for enhanced tissue integration.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
616.3 - Patología del aparato digestivo. Odontología
Palabras clave
Hidrogels basats en nucleòsids
Guanosina i derivats
Impressió 3D
Polidopamina
Activitat antibacteriana
Hidrogeles a base de nucleósidos
Guanosina y derivados
Impresión 3D
Polidopamina
Actividad antibacterial
Nucleoside-based hydrogels
Guanosine and derivatives
3D printing
Polydopamine
Antibacterial activity
Páginas
25
Publicado por
MDPI
Colección
24; 4
Publicado en
International Journal of Molecular Sciences
Citación
Merino Gómez, Maria; Gil, FJ; Perez, Roman [et al.]. Polydopamine incorporation enhances cell differentiation and antibacterial properties of 3D-printed guanosine-borate hydrogels for functional tissue regeneration. International Journal of Molecular Sciences, 2023, 24(4), 4224. Disponible en: <https://www.mdpi.com/1422-0067/24/4/4224>. Fecha de acceso: 27 mar. 2023. DOI: 10.3390/ijms24044224
Enlace al documento relacionado
Este ítem aparece en la(s) siguiente(s) colección(ones)
- Odontologia [245]
Derechos
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Excepto si se señala otra cosa, la licencia del ítem se describe como https://creativecommons.org/licenses/by/4.0/