dc.contributor.author | Qi, Hongyun | |
dc.contributor.author | Wright, Roni | |
dc.contributor.author | Beato, Miguel | |
dc.contributor.author | Price, Brendan D. | |
dc.date.accessioned | 2023-03-01T09:18:13Z | |
dc.date.available | 2023-03-01T09:18:13Z | |
dc.date.issued | 2022-12 | |
dc.identifier.citation | Qi, Hongyun; Wright, Roni; Beato, Miguel [et al.]. The ADP-ribose hydrolase NUDT5 is important for DNA repair. Cell Reports, 2022, 41(12), 111866. Disponible en: <https://www.sciencedirect.com/science/article/pii/S2211124722017624?via%3Dihub>. Fecha de acceso: 1 mar. 2023. DOI: 10.1016/j.celrep.2022.111866 | ca |
dc.identifier.issn | 2639-1856 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/3589 | |
dc.description.abstract | DNA damage leads to rapid synthesis of poly(ADP-ribose) (pADPr), which is important for damage signaling and repair. pADPr chains are removed by poly(ADP-ribose) glycohydrolase (PARG), releasing free mono(ADP-ribose) (mADPr). Here, we show that the NUDIX hydrolase NUDT5, which can hydrolyze mADPr to ribose-5-phosphate and either AMP or ATP, is recruited to damage sites through interaction with PARG. NUDT5 does not regulate PARP or PARG activity. Instead, loss of NUDT5 reduces basal cellular ATP levels and exacerbates the decrease in cellular ATP that occurs during DNA repair. Further, loss of NUDT5 activity impairs RAD51 recruitment, attenuates the phosphorylation of key DNA-repair proteins, and reduces both H2A.Z exchange at damage sites and repair by homologous recombination. The ability of NUDT5 to hydrolyze mADPr, and/or regulate cellular ATP, may therefore be important for efficient DNA repair. Targeting NUDT5 to disrupt PAR/mADPr and energy metabolism may be an effective anti-cancer strategy. | en |
dc.format.extent | 13 | ca |
dc.language.iso | eng | ca |
dc.publisher | Springer Nature | ca |
dc.relation.ispartof | Cell Reports | ca |
dc.relation.ispartofseries | 41;12 | |
dc.relation.uri | https://www.sciencedirect.com/science/article/pii/S2211124722017624?via%3Dihub | ca |
dc.rights | Under a Creative Commons license. | en |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.other | NUDT5 | ca |
dc.subject.other | NUDIX hidrolasa | ca |
dc.subject.other | Reparació de l'ADN | ca |
dc.subject.other | Metabolisme energètic | ca |
dc.subject.other | ATP | ca |
dc.subject.other | PARPs | ca |
dc.subject.other | Mono (ADP-ribosa) | ca |
dc.subject.other | Recombinació homòloga | ca |
dc.subject.other | Cromatina | ca |
dc.subject.other | NUDT5 | es |
dc.subject.other | NUDIX hidrolasa | es |
dc.subject.other | Reparación de ADN | es |
dc.subject.other | Metabolismo energético | es |
dc.subject.other | ATP | es |
dc.subject.other | PARP | es |
dc.subject.other | Mono(ADP-ribosa) | es |
dc.subject.other | Recombinación homóloga | es |
dc.subject.other | Cromatina | es |
dc.subject.other | NUDT5 | en |
dc.subject.other | NUDIX hydrolase | en |
dc.subject.other | DNA repair | en |
dc.subject.other | Energy metabolism | en |
dc.subject.other | ATP | en |
dc.subject.other | PARPs | en |
dc.subject.other | Mono(ADP-ribose) | en |
dc.subject.other | Homologous recombination | en |
dc.subject.other | Chromatin | en |
dc.title | The ADP-ribose hydrolase NUDT5 is important for DNA repair | en |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 57 | ca |
dc.identifier.doi | https://dx.doi.org/10.1016/j.celrep.2022.111866 | ca |